University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Genetic and phenotypic intrastrain variation in herpes simplex virus type 1 Glasgow strain 17 syn+ derived viruses

Jones, Juliet, Depledge, Daniel Pearce, Breuer, Judith, Ebert-Keel, Katja and Elliott, Gillian (2019) Genetic and phenotypic intrastrain variation in herpes simplex virus type 1 Glasgow strain 17 syn+ derived viruses Journal of General Virology.

[img]
Preview
Text
Genetic and phenotypic intrastrain variation in herpes simplex virus type 1 Glasgow strain 17 syn+ derived viruses.pdf - Accepted version Manuscript
Available under License Creative Commons Attribution.

Download (6MB) | Preview

Abstract

The Glasgow s17 syn+ strain of herpes simplex virus 1 (HSV1) is arguably the best characterised strain and has provided the reference sequence for HSV1 genetic studies. Here we show that our original s17 syn+ stock was a mixed population from which we have isolated a minor variant that, unlike other strains in the laboratory, fails to be efficiently released from infected cells and spreads predominantly by direct cell-to-cell transmission. Analysis of other s17-derived viruses that had been isolated elsewhere revealed a number with the same release phenotype. Second generation sequencing of eight plaque-purified s17-derived viruses revealed sequences that vary by 50 SNPs including approximately 10 coding SNPs. This compared to interstrain variations of around 800 SNPs in strain Sc16, of which a quarter were coding changes. Amongst the variations found within s17, we identified thirteen variants of glycoprotein C within the original stock of virus which were predominantly a consequence of altered homopolymeric runs of C residues. Characterisation of seven isolates coding for different forms of gC indicated that all were expressed, despite six of them lacking a transmembrane domain. While the release phenotype did not correlate directly with any of these identified gC variations, further demonstration that nine clinical isolates of HSV1 also fail to spread through extracellular release raises the possibility that propagation in tissue culture had altered the HSV1 s17 transmission phenotype. Hence, this s17 intrastrain variation identified here offers an excellent model for understanding both HSV1 transmission and tissue culture adaptation.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Jones, Julietj.l.jones@surrey.ac.uk
Depledge, Daniel Pearce
Breuer, Judith
Ebert-Keel, Katjak.ebert-keel@surrey.ac.uk
Elliott, Gilliang.elliott@surrey.ac.uk
Date : 2019
Funders : Worldwide Cancer Research
Copyright Disclaimer : © 2019 The Authors.
Related URLs :
Depositing User : Clive Harris
Date Deposited : 04 Oct 2019 13:45
Last Modified : 04 Oct 2019 13:45
URI: http://epubs.surrey.ac.uk/id/eprint/852880

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800