University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Blue light (λ=453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in humans in-vivo

Albers, Isabel, Zernickel, Erika, Stern, Manuel, Broja, Melanie, Hans Lucas, Busch, Heiss, Christian, Grotheer, Vera, Windolf, Joachim and Suschek, Christoph V. (2019) Blue light (λ=453 nm) nitric oxide dependently induces β-endorphin production of human skin keratinocytes in-vitro and increases systemic β-endorphin levels in humans in-vivo Free Radical Biology and Medicine, 145. pp. 78-86.

[img] Text
Blue light.pdf - Accepted version Manuscript
Restricted to Repository staff only until 22 September 2020.

Download (2MB)


β-Endorphin exerts a broad spectrum of physiological activity on mood, immune functions, pain management, reward effects, and behavioral stability. β-Endorphin is produced in certain neurons within the central and peripheral nervous system but also in the skin, especially in response to ultraviolet radiation. In the present study we have investigated the impact of visible blue light at λ = 453 nm (BL) on β-endorphin production of primary human skin keratinocytes (hKC) in-vitro as well as on systemic β-endorphin formation of whole-body exposed subjects in-vivo. We found that BL irradiation significantly enhanced both keratinocytic β-endorphin production of hKC cultures as well as systemic β-endorphin concentrations in light exposed healthy subjects. Interestingly, in hKC cultures elevated β-endorphin formation was paralleled by significantly increased levels of non-enzymatically generated nitric oxide (NO), whereas elevated systemic β-endorphin values of BL-exposed subjects were accompanied by enhanced systemic concentration of bioactive NO-derivates. These findings point to a pivotal role of NO in the molecular mechanism of the observed BL-induced effects, and indeed, exogenously applied NO was able to significantly enhance β-endorphin production in hKC cultures. Thus, our finding of BL-induced increases in systemic β-endorphin concentration in-vivo can be plausibly explained by an event sequence comprising 1.) BL-driven non-enzymatic formation of NO in the exposed skin tissue, 2.) systemic distribution of cutaneously produced NO in the Form of bioactive nitroso compounds, 3.) a subsequent NO-dependent induction of β-endorphin synthesis in epidermal keratinocytes, and 4.) probably also a NO-dependent modulation of β-endorphin synthesis in specialized neurons within the central and peripheral nervous system.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Albers, Isabel
Zernickel, Erika
Stern, Manuel
Broja, Melanie
Hans Lucas, Busch
Grotheer, Vera
Windolf, Joachim
Suschek, Christoph V.
Date : December 2019
Funders : Philips
DOI : 10.1016/j.freeradbiomed.2019.09.022
Copyright Disclaimer : © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Uncontrolled Keywords : Nitric oxide; Beta-endorphin; Blue light; Keratinocytes; POMC; In vivo study
Depositing User : Clive Harris
Date Deposited : 01 Oct 2019 08:32
Last Modified : 21 Nov 2019 13:00

Actions (login required)

View Item View Item


Downloads per month over past year

Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800