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Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells

Beale, Andrew D., Kruchek, Emily, Kitcatt, Stephen J., Henslee, Erin A., Parry, Jack S.W., Braun, Gabriella, Jabr, Rita, von Schantz, Malcolm, O’Neill, John S. and Labeed, Fatima (2019) Casein Kinase 1 Underlies Temperature Compensation of Circadian Rhythms in Human Red Blood Cells Journal of Biological Rhythms, 34 (2). pp. 144-153.

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Abstract

Temperature compensation and period determination by casein kinase 1 (CK1) are conserved features of eukaryotic circadian rhythms, whereas the clock gene transcription factors that facilitate daily gene expression rhythms differ between phylogenetic kingdoms. Human red blood cells (RBCs) exhibit temperature-compensated circadian rhythms, which, because RBCs lack nuclei, must occur in the absence of a circadian transcription-translation feedback loop. We tested whether period determination and temperature compensation are dependent on CKs in RBCs. As with nucleated cell types, broad-spectrum kinase inhibition with staurosporine lengthened the period of the RBC clock at 37°C, with more specific inhibition of CK1 and CK2 also eliciting robust changes in circadian period. Strikingly, inhibition of CK1 abolished temperature compensation and increased the Q10 for the period of oscillation in RBCs, similar to observations in nucleated cells. This indicates that CK1 activity is essential for circadian rhythms irrespective of the presence or absence of clock gene expression cycles.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Beale, Andrew D.a.beale@surrey.ac.uk
Kruchek, Emilye.j.kruchek@surrey.ac.uk
Kitcatt, Stephen J.stephen.kitcatt@surrey.ac.uk
Henslee, Erin A.e.henslee@surrey.ac.uk
Parry, Jack S.W.
Braun, Gabriella
Jabr, Ritarita.jabr@surrey.ac.uk
von Schantz, MalcolmM.Von.Schantz@surrey.ac.uk
O’Neill, John S.
Labeed, FatimaF.Labeed@surrey.ac.uk
Date : 1 April 2019
Funders : Biotechnology and Biological Sciences Research Council (BBSRC), Medical Research Council (MRC)
DOI : 10.1177/0748730419836370
Copyright Disclaimer : This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
Uncontrolled Keywords : Casein kinase; Temperature compensation; Erythrocyte; Dielectrophoresis; Electrophysiology
Depositing User : Clive Harris
Date Deposited : 30 Sep 2019 10:00
Last Modified : 30 Sep 2019 10:00
URI: http://epubs.surrey.ac.uk/id/eprint/852833

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