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DNA DSB Repair Dynamics following Irradiation with Laser-Driven Protons at Ultra-High Dose Rates

Hanton, F., Chaudhary, P., Doria, D., Gwynne, D., Maiorino, C., Scullion, C., Ahmed, H., Marshall, T., Naughton, K., Romagnani, L. , Kar, S., Schettino, G., McKenna, P., Botchway, S., Symes, D. R., Rajeev, P. P., Prise, K. M. and Borghesi, M. (2019) DNA DSB Repair Dynamics following Irradiation with Laser-Driven Protons at Ultra-High Dose Rates Scientific Reports, 9, 4471. pp. 1-10.

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Abstract

Protontherapy has emerged as more effective in the treatment of certain tumors than photon based therapies. However, significant capital and operational costs make protontherapy less accessible. This has stimulated interest in alternative proton delivery approaches, and in this context the use of laser-based technologies for the generation of ultra-high dose rate ion beams has been proposed as a prospective route. A better understanding of the radiobiological effects at ultra-high dose-rates is important for any future clinical adoption of this technology. In this study, we irradiated human skin fibroblasts-AG01522B cells with laser-accelerated protons at a dose rate of 109 Gy/s, generated using the Gemini laser system at the Rutherford Appleton Laboratory, UK. We studied DNA double strand break (DSB) repair kinetics using the p53 binding protein-1(53BP1) foci formation assay and observed a close similarity in the 53BP1 foci repair kinetics in the cells irradiated with 225 kVp X-rays and ultra- high dose rate protons for the initial time points. At the microdosimetric scale, foci per cell per track values showed a good correlation between the laser and cyclotron-accelerated protons indicating similarity in the DNA DSB induction and repair, independent of the time duration over which the dose was delivered.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Physics
Authors :
NameEmailORCID
Hanton, F.
Chaudhary, P.
Doria, D.
Gwynne, D.
Maiorino, C.
Scullion, C.
Ahmed, H.
Marshall, T.
Naughton, K.
Romagnani, L.
Kar, S.
Schettino, G.giuseppe.schettino@surrey.ac.uk
McKenna, P.
Botchway, S.
Symes, D. R.
Rajeev, P. P.
Prise, K. M.
Borghesi, M.
Date : 14 March 2019
Funders : Engineering and Physical Sciences Research Council (EPSRC)
DOI : 10.1038/s41598-019-40339-6
Grant Title : Advanced Strategies for Accelerating Ions with Laser (ASAIL)
Copyright Disclaimer : © The Author(s) 2019 Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Depositing User : Clive Harris
Date Deposited : 24 Sep 2019 15:28
Last Modified : 24 Sep 2019 15:28
URI: http://epubs.surrey.ac.uk/id/eprint/852776

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