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Isolation and characterisation of alveolar type II pneumocytes from adult bovine lung

Lee, Diane Frances, Salguero, Francisco Javier, Grainger, Duncan, Francis, Robert James, MacLellan-Gibson, Kirsty and Chambers, Mark Andrew (2018) Isolation and characterisation of alveolar type II pneumocytes from adult bovine lung Scientific Reports, 8 (1).

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Abstract

Alveolar type II (ATII) cells play a key role as part of the distal lung epithelium, including roles in the innate immune response and as self-renewing progenitors to replace alveolar type I (ATI) cells during regeneration of the alveolar epithelium. Their secretion of surfactant protein helps to maintain homeostasis in the distal lung and exert protective, antimicrobial properties. Despite the cell’s crucial roles, they remain difficult to study, in part due to inefficient and expensive isolation methods, a propensity to differentiate into alveolar type I cells in culture and susceptibility to fibroblast overgrowth from primary isolations. Published methods of isolation often require specialist technology, negatively impacting the development of in vitro models of disease, including bovine tuberculosis (BTB), a serious re-emerging disease in both animals and humans worldwide. We present here a simple and cost effective method that may be utilised in the generation of bovine primary ATII cells. These exhibit an ATII phenotype in 2D and 3D culture in our studies and are conducive to further study of the role of ATII cells in bovine respiratory diseases.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Lee, Diane Francesdiane.lee@surrey.ac.uk
Salguero, Francisco Javierf.salguerobodes@surrey.ac.uk
Grainger, Duncand.grainger@surrey.ac.uk
Francis, Robert James
MacLellan-Gibson, Kirsty
Chambers, Mark Andrewm.chambers@surrey.ac.uk
Date : 9 August 2018
Funders : The National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs)
DOI : 10.1038/s41598-018-30234-x
Copyright Disclaimer : © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Depositing User : Diane Maxfield
Date Deposited : 03 Sep 2019 14:14
Last Modified : 03 Sep 2019 14:14
URI: http://epubs.surrey.ac.uk/id/eprint/852535

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