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Isolation of Alveolar Type II Cells from Adult Bovine Lung

Lee, Diane Frances and Chambers, Mark Andrew (2019) Isolation of Alveolar Type II Cells from Adult Bovine Lung Current Protocols in Toxicology, 80 (1), e71.

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Abstract

Alveolar type II (ATII) cells play a key role as part of the distal lung epithelium, including in the innate immune response and as self‐renewing progenitors to replace alveolar type I (ATI) cells during epithelial regeneration. Their secretion of surfactant protein helps maintain homeostasis and exerts protective, antimicrobial properties. ATII cells remain difficult to study, partly due to inefficient and expensive isolation methods, a propensity to differentiate into ATI cells, and susceptibility to fibroblast contamination. Published methods of isolation often require specialized technology, negatively impacting the development of in vitro models of disease, including bovine tuberculosis. Presented here is a simple and cost‐effective method for generation of bovine primary ATII cells. These cells exhibit an ATII phenotype in 2D and 3D culture and are conducive to further study of the role of ATII cells in bovine respiratory diseases.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Lee, Diane Francesdiane.lee@surrey.ac.uk
Chambers, Mark Andrewm.chambers@surrey.ac.uk
Date : 18 June 2019
Funders : National Centre for the Replacement, Refinement and Reduction of Animals in Research
DOI : 10.1002/cptx.71
Grant Title : NC3Rs
Copyright Disclaimer : © 2019 by John Wiley & Sons, Inc.
Uncontrolled Keywords : Alveolus; ATII; Bovine; Epithelial: Isolation
Additional Information : This article also appears in: Respiratory Toxicology https://currentprotocols.onlinelibrary.wiley.com/doi/toc/10.1002/(ISSN)1934-9262.RespiratoryToxicology
Depositing User : Diane Maxfield
Date Deposited : 03 Sep 2019 13:17
Last Modified : 05 Sep 2019 12:02
URI: http://epubs.surrey.ac.uk/id/eprint/852531

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