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Secretion and functional expression of Mycobacterium bovis antigens MPB70 and MPB83 in lactic acid bacteria

Stedman, Anna, Chambers, Mark A. and Gutierrez-Merino, Jorge (2019) Secretion and functional expression of Mycobacterium bovis antigens MPB70 and MPB83 in lactic acid bacteria TUBERCULOSIS, 117. pp. 24-30.

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Abstract

The aim of this study was to determine the reliability of lactic acid bacteria (LAB) as heterologous hosts for the expression of MPB70 and MPB83, two Mycobacterium bovis antigens that possess diagnostics and immunogenic properties, respectively. We therefore generated recombinant cells of Lactococcus lactis and Lactobacillus plantarum that carried hybrid genes encoding MPB70 and MPB83 fused to signal peptides that are specifically recognized by LAB. Only L. lactis was able to secrete MPB70 using the L. lactis signal peptide Usp45, and to produce MPB83 as an immunogenic membrane protein following its expression with the signal peptide of the L. plantarum lipoprotein prsA. Inactivated cells of MPB83-expressing L. lactis cultures enhanced NF-κB activation in macrophages. Our results show that L. lactis is a reliable host for the secretion and functional expression of antigens that are naturally produced by M. bovis, the causative agent of bovine tuberculosis (bTB). This represents the first step on a long process to establishing whether recombinant LAB could serve as a food-grade platform for potential diagnostic tools and/or vaccine interventions for use against bTB, a chronic disease that primarily affects cattle but also humans and a wide range of domestic and wild animals.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Stedman, Anna
Chambers, Mark A.m.chambers@surrey.ac.uk
Gutierrez-Merino, Jorgej.gutierrez@surrey.ac.uk
Date : July 2019
Funders : Morris Animal Foundation
DOI : 10.1016/j.tube.2019.05.007
Copyright Disclaimer : © 2019 Published by Elsevier Ltd.
Uncontrolled Keywords : Lactic acid bacteria; Mycobacterium bovis; Antigen; Functional expression; Secretion; Bovine tuberculosis
Depositing User : Diane Maxfield
Date Deposited : 02 Sep 2019 11:08
Last Modified : 02 Sep 2019 11:08
URI: http://epubs.surrey.ac.uk/id/eprint/852520

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