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A multi-scale analysis of the effect of complex viscoelastic models on Listeria dynamics and adaptation in co-culture systems

Costello, Katherine M., Gutierrez-Merino, Jorge, Bussemaker, Madeleine, Smet, Cindy, Van Impe, Jan F. and Velliou, Eirini G. (2020) A multi-scale analysis of the effect of complex viscoelastic models on Listeria dynamics and adaptation in co-culture systems AIChE Journal, 66 (1), e16761.

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Abstract

Natural antimicrobials are of interest to replace traditional food decontamination methods: they are milder and maintain desirable sensory characteristics. However, efficacy can be affected by food structure/composition, thus structural effects in a co-culture pathogen/microflora system are investigated. Listeria was grown planktonically (liquid broth) or on a biphasic viscoelastic system, in monoculture with/without artificial nisin, or in co-culture with L. lactis (nisin/non-nisin producing). Microbial growth kinetics were monitored and advanced microscopy techniques were utilised to quantify cellular interactions and spatial organisation.

Microstructural effects are observed on the kinetics, with differences in monoculture/co- culture. Significant microscopic differences are observed in spatial organisation and colony size. We are the first to observe changing growth location for all species in monoculture/co- culture, with differences in colony size/organisation through stationary phase. This study provides insight into the environmental stress response/adaptation of Listeria grown on structured systems in response to L. lactis and natural antimicrobials.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :
NameEmailORCID
Costello, Katherine M.k.costello@surrey.ac.uk
Gutierrez-Merino, Jorgej.gutierrez@surrey.ac.uk
Bussemaker, Madeleinem.bussemaker@surrey.ac.uk
Smet, Cindy
Van Impe, Jan F.
Velliou, Eirini G.e.velliou@surrey.ac.uk
Date : January 2020
Funders : Royal Society, IAA-EPSRC
DOI : 10.1002/aic.16761
Copyright Disclaimer : This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2019 The Authors. AIChE Journal published by Wiley Periodicals, Inc. on behalf of American Institute of Chemical Engineers.
Uncontrolled Keywords : Listeria; Stress adaptation; Microscopy; Co-culture; Food microstructure
Related URLs :
Depositing User : Clive Harris
Date Deposited : 22 Aug 2019 08:05
Last Modified : 18 Dec 2019 11:36
URI: http://epubs.surrey.ac.uk/id/eprint/852459

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