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Domestication of Campylobacter jejuni NCTC 11168

Pascoe, Ben, Williams, Lisa K., Calland, Jessica K., Meric, Guillaume, Hitchings, Matthew D., Dyer, Myles, Ryder, Joseph, Shaw, Sophie, Lopes, Bruno S., Chintoan-Uta, Cosmin , Allan, Elaine, Vidal, Ana, Fearnley, Catherine, Everest, Paul, Pachebat, Justin A., Cogan, Tristan A., Stevens, Mark P., Humphrey, Thomas J., Wilkinson, Thomas S., Cody, Alison J., Colles, Frances M., Jolley, Keith A., Maiden, Martin C. J., Strachan, Norval, Pearson, Bruce M., Linton, Dennis, Wren, Brendan W., Parkhill, Julian, Kelly, David J, van Vliet, Arnoud H. M., Forbes, Ken J. and Sheppard, Samuel K. (2019) Domestication of Campylobacter jejuni NCTC 11168 Microbial Genomics.

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Abstract

Reference and type strains of well-known bacteria have been a cornerstone of microbiology research for decades. The sharing of well-characterized isolates among laboratories has run in parallel with research efforts and enhanced the reproducibility of experiments, leading to a wealth of knowledge about trait variation in different species and the underlying genetics. Campylobacter jejuni strain NCTC 11168, deposited at the National Collection of Type Cultures in 1977, has been adopted widely as a reference strain by researchers worldwide and was the first Campylobacter for which the complete genome was published (in 2000). In this study, we collected 23 C . jejuni NCTC 11168 reference isolates from laboratories across the UK and compared variation in simple laboratory phenotypes with genetic variation in sequenced genomes. Putatively identical isolates, identified previously to have aberrant phenotypes, varied by up to 281 SNPs (in 15 genes) compared to the most recent reference strain. Isolates also display considerable phenotype variation in motility, morphology, growth at 37 °C, invasion of chicken and human cell lines, and susceptibility to ampicillin. This study provides evidence of ongoing evolutionary change among C. jejuni isolates as they are cultured in different laboratories and highlights the need for careful consideration of genetic variation within laboratory reference strains. This article contains data hosted by Microreact.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Pascoe, Ben
Williams, Lisa K.
Calland, Jessica K.
Meric, Guillaume
Hitchings, Matthew D.
Dyer, Myles
Ryder, Joseph
Shaw, Sophie
Lopes, Bruno S.
Chintoan-Uta, Cosmin
Allan, Elaine
Vidal, Ana
Fearnley, Catherine
Everest, Paul
Pachebat, Justin A.
Cogan, Tristan A.
Stevens, Mark P.
Humphrey, Thomas J.
Wilkinson, Thomas S.
Cody, Alison J.
Colles, Frances M.
Jolley, Keith A.
Maiden, Martin C. J.
Strachan, Norval
Pearson, Bruce M.
Linton, Dennis
Wren, Brendan W.
Parkhill, Julian
Kelly, David J
van Vliet, Arnoud H. M.a.vanvliet@surrey.ac.uk
Forbes, Ken J.
Sheppard, Samuel K.
Date : 16 July 2019
Funders : Medical Research Council (MRC)
DOI : 10.1099/mgen.0.000279
Copyright Disclaimer : © 2019 The Authors. Published by the Microbiology Society. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords : Genomics; Microbial evolution; Culture collections; Campylobacter
Depositing User : Clive Harris
Date Deposited : 25 Jul 2019 10:43
Last Modified : 25 Jul 2019 10:43
URI: http://epubs.surrey.ac.uk/id/eprint/852307

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