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Transposon libraries identify novel Mycobacterium bovis BCG genes involved in the dynamic interactions required for BCG to persist during in vivo passage in cattle

Mendum, Tom A., Chandran, Aneesh, Williams, Kerstin, Vordermeier, H. Martin, Villarreal-Ramos, Bernardo, Wu, H., Singh, Albel, Smith, Alex A., Butler, Rachel E., Prasad, Aravind , Bharti, Neeraj, Banerjee, Ruma, Kasibhatla, Sunitha M., Bhatt, Apoorva, Stewart, Graham R. and McFadden, Johnjoe (2019) Transposon libraries identify novel Mycobacterium bovis BCG genes involved in the dynamic interactions required for BCG to persist during in vivo passage in cattle BMC Genomics, 20, 431. pp. 1-13.

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Abstract

Background

BCG is the most widely used vaccine of all time and remains the only licensed vaccine for use against tuberculosis in humans. BCG also protects other species such as cattle against tuberculosis, but due to its incompatibility with current tuberculin testing regimens remains unlicensed. BCG’s efficacy relates to its ability to persist in the host for weeks, months or even years after vaccination. It is unclear to what degree this ability to resist the host’s immune system is maintained by a dynamic interaction between the vaccine strain and its host as is the case for pathogenic mycobacteria.

Results

To investigate this question, we constructed transposon mutant libraries in both BCG Pasteur and BCG Danish strains and inoculated them into bovine lymph nodes. Cattle are well suited to such an assay, as they are naturally susceptible to tuberculosis and are one of the few animal species for which a BCG vaccination program has been proposed. After three weeks, the BCG were recovered and the input and output libraries compared to identify mutants with in vivo fitness defects. Less than 10% of the mutated genes were identified as affecting in vivo fitness, they included genes encoding known mycobacterial virulence functions such as mycobactin synthesis, sugar transport, reductive sulphate assimilation, PDIM synthesis and cholesterol metabolism. Many other attenuating genes had not previously been recognised as having a virulence phenotype. To test these genes, we generated and characterised three knockout mutants that were predicted by transposon mutagenesis to be attenuating in vivo: pyruvate carboxylase, a hypothetical protein (BCG_1063), and a putative cyclopropane-fatty-acyl-phospholipid synthase. The knockout strains survived as well as wild type during in vitro culture and in bovine macrophages, yet demonstrated marked attenuation during passage in bovine lymph nodes confirming that they were indeed involved in persistence of BCG in the host.

Conclusion

These data show that BCG is far from passive during its interaction with the host, rather it continues to employ its remaining virulence factors, to interact with the host’s innate immune system to allow it to persist, a property that is important for its protective efficacy.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Mendum, Tom A.T.Mendum@surrey.ac.uk
Chandran, Aneeshaneesh.chandran@surrey.ac.uk
Williams, Kerstink.j.williams@surrey.ac.uk
Vordermeier, H. Martin
Villarreal-Ramos, Bernardo
Wu, H.
Singh, Albel
Smith, Alex A.alex.smith@surrey.ac.uk
Butler, Rachel E.R.Shrimpton@surrey.ac.uk
Prasad, Aravind
Bharti, Neeraj
Banerjee, Ruma
Kasibhatla, Sunitha M.
Bhatt, Apoorva
Stewart, Graham R.G.Stewart@surrey.ac.uk
McFadden, JohnjoeJ.Mcfadden@surrey.ac.uk
Date : 28 May 2019
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
DOI : 10.1186/s12864-019-5791-1
Copyright Disclaimer : © The Author(s). 2019 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Uncontrolled Keywords : Mycobacterium bovis BCG; Transposon library; Cattle; Mycobacterium tuberculosis; Pyruvate carboxylase; Cyclopropane-fatty-acyl-phospholipid synthase; Rv1006
Depositing User : Clive Harris
Date Deposited : 11 Jun 2019 08:27
Last Modified : 11 Jun 2019 08:27
URI: http://epubs.surrey.ac.uk/id/eprint/851968

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