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A novel versatile animal-free 3D tool for rapid low-cost assessment of immunodiagnostic microneedles

Totti, Stella, Ng, Keng Wooi, Dale, Lorraine, Lian, Guoping, Chen, Tao and Velliou, Eirini G. (2019) A novel versatile animal-free 3D tool for rapid low-cost assessment of immunodiagnostic microneedles Sensors and Actuators B: Chemical, 296, 126652. pp. 1-8.

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Abstract

Microneedle devices offer minimally invasive and rapid biomarker extraction from the skin. However, the lack of effective assessment tools for such microneedle devices can delay their development into useful clinical applications. Traditionally, the microneedle performance is evaluated i) in vivo, using animal models, ii) ex vivo, on excised human or animal skin or iii) in vitro, using homogenised solutions with the target antigen to model the interstitial fluid. In vivo and ex vivo models are considered the gold-standard approach for the evaluation of microneedle devices because of their structural composition, however they do exhibit limitations. More specifically, they have limited availability and they present batch-to-batch variations depending on the skin origin. Furthermore, their use rises ethical concerns regarding compliance with the globally accepted 3Rs principle of reducing the use of animals for research purposes. At the same time, in vitro models fail to accurately mimic the structure and the mechanical integrity of the skin tissue that surrounds the interstitial fluid. In this study, we introduce for the first time an animal-free, mechanically robust, 3D scaffold that has great potential as an accurate in vitro evaluation tool for immunodiagnostic microneedle devices. More specifically, we demonstrate, for the first time, successful extraction and detection of a melanoma biomarker (S100B) using immunodiagnostic microneedles in the 3D culture system. Melanoma cells (A375) were cultured and expanded for 35 days in the highly porous polymeric scaffold followed by in situ capture of S100B with the microneedle device. Scanning electron microscopy showed a close resemblance between the 3D scaffold and human skin in terms of internal structure and porosity. The microneedle device detected S100B in the scaffold (with a detection pattern similar to the positive controls), while the biomarker was not detected in the surrounding liquid supernatants. Our findings demonstrate the great potential of this animal-free 3D tool for rapid and low-cost evaluation of microneedle devices.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :
NameEmailORCID
Totti, Stellastyliani.totti@surrey.ac.uk
Ng, Keng Wooi
Dale, Lorraine
Lian, Guopingg.lian@surrey.ac.uk
Chen, TaoT.Chen@surrey.ac.uk
Velliou, Eirini G.e.velliou@surrey.ac.uk
Date : 1 October 2019
Funders : University of Surrey, Engineering and Physical Sciences Research Council (EPSRC)
DOI : 10.1016/j.snb.2019.126652
Copyright Disclaimer : © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Microneedles; Diagnostic device; Biomarker detection; S100B; Melanoma; Three-dimensional in vitro model
Depositing User : Clive Harris
Date Deposited : 10 Jun 2019 08:14
Last Modified : 15 Oct 2019 12:33
URI: http://epubs.surrey.ac.uk/id/eprint/851960

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