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Molecular and in vitro characterisation of hepatitis E virus from UK pigs

Grierson, Sylvia S., Mcgowan, Sarah, Cook, Charlotte, Steinbach, Falko and Choudhury, Bhudipa (2019) Molecular and in vitro characterisation of hepatitis E virus from UK pigs Virology, 527. pp. 116-121.

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Molecular and in vitro characterisation of hepatitis E virus from UK pigs.doc - Accepted version Manuscript

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Abstract

Hepatitis E virus (HEV) infection is widespread in the global pig population. Although clinically inapparent in pigs, HEV infection is the cause of Hepatitis E in humans and transmission via the food chain has been established. Following a 2013 study that investigated prevalence of HEV infection in UK slaughter-age pigs samples indicating highest viral load were selected for further characterisation. High throughput sequencing was used to obtain the complete coding sequence from five samples. An in-frame insertion was observed within the HEV hypervariable region in two samples. To interrogate whether this mutation may be the cause of high-level viraemia and faecal shedding as observed in the sampled pigs virus isolation and culture was conducted. Based on viral growth kinetics there was no evidence that these insertions affected replication efficiency in vitro, suggesting as yet undetermined host factors may affect the course of infection and consequently the risk of foodborne transmission.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Grierson, Sylvia S.
Mcgowan, Sarahs.mcgowan@surrey.ac.uk
Cook, Charlotte
Steinbach, Falkof.steinbach@surrey.ac.uk
Choudhury, Bhudipa
Date : 15 January 2019
Funders : Department for Environment, Food and Rural Affairs
DOI : 10.1016/j.virol.2018.10.018
Copyright Disclaimer : © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Hepatitis E virus; Pigs; UK; Genotype 3; Complete coding sequence; Virus isolates
Depositing User : Clive Harris
Date Deposited : 10 May 2019 07:31
Last Modified : 27 Nov 2019 02:08
URI: http://epubs.surrey.ac.uk/id/eprint/851782

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