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Dietary supplementation with inulin-propionate ester or inulin improves insulin sensitivity in adults with overweight and obesity with distinct effects on the gut microbiota, plasma metabolome and systemic inflammatory responses: a randomised crossover trial

Chambers, Edward S., Byrne, Claire S., Morrison, Douglas J., Murphy, Kevin G., Preston, Tom, Tedford, M. Catriona, Garcia-Perez, Isabel, Fountana, Sofia, Serrano-Contreras, Jose I., Holmes, Elaine , Roberts, Jordie F., Reynolds, Catherine J., Boyton, Rosemary J., Altmann, Daniel M., McDonald, Julie A.K., Marchesi, Julian R., Akbar, Arne N., Riddell, Natalie E., Wallis, Gareth A. and Frost, Gary (2019) Dietary supplementation with inulin-propionate ester or inulin improves insulin sensitivity in adults with overweight and obesity with distinct effects on the gut microbiota, plasma metabolome and systemic inflammatory responses: a randomised crossover trial Gut.

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Abstract

Objective: To investigate the underlying mechanisms behind changes in glucose homeostasis with delivery of propionate to the human colon by comprehensive and coordinated analysis of gut bacterial composition, plasma metabolome and immune responses.

Design: Twelve non-diabetic adults with overweight and obesity received 20g/day of inulin-propionate ester (IPE), designed to selectively deliver propionate to the colon, a high-fermentable fibre control (inulin) and a low-fermentable fibre control (cellulose) in a randomised, double-blind, placebo controlled, crossover design. Outcome measurements of metabolic responses, inflammatory markers and gut bacterial composition were analysed at the end of each 42-day supplementation period.

Results: Both IPE and inulin supplementation improved insulin resistance compared to cellulose supplementation, measured by homeostatic model assessment (HOMA) 2 (Mean±SEM 1.23±0.17 IPE vs. 1.59±0.17 cellulose, P=0.001; 1.17±0.15 inulin vs. 1.59±0.17 cellulose, P=0.009), with no differences between IPE and inulin (P=0.272). Fasting insulin was only associated positively with plasma tyrosine and negatively with plasma glycine following inulin supplementation. IPE supplementation decreased pro-inflammatory IL-8 levels compared to cellulose, whilst inulin had no impact on the systemic inflammatory markers studied. Inulin promoted changes in gut bacterial populations at the class level (increased Actinobacteria and decreased Clostridia) and order level (decreased Clostridales) compared to cellulose, with small differences at the species level observed between IPE and cellulose.

Conclusion: These data demonstrate a distinctive physiological impact of raising colonic propionate delivery in humans, as improvements in insulin sensitivity promoted by IPE and inulin were accompanied with different effects on the plasma metabolome, gut bacterial populations and markers of systemic inflammation.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Chambers, Edward S.
Byrne, Claire S.
Morrison, Douglas J.
Murphy, Kevin G.
Preston, Tom
Tedford, M. Catriona
Garcia-Perez, Isabel
Fountana, Sofia
Serrano-Contreras, Jose I.
Holmes, Elaine
Roberts, Jordie F.
Reynolds, Catherine J.
Boyton, Rosemary J.
Altmann, Daniel M.
McDonald, Julie A.K.
Marchesi, Julian R.
Akbar, Arne N.
Riddell, Natalie E.n.riddell@surrey.ac.uk
Wallis, Gareth A.
Frost, Gary
Date : 2019
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
Copyright Disclaimer : © Author(s) (or their employer(s)) 2019. No commercial re-use. See rights and permissions. Published by BMJ.
Uncontrolled Keywords : Short Chain Fatty Acids; Glucose Metabolism; Colonic Microflora; Inflammation
Related URLs :
Depositing User : Clive Harris
Date Deposited : 07 Mar 2019 08:20
Last Modified : 07 Mar 2019 08:23
URI: http://epubs.surrey.ac.uk/id/eprint/850681

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