University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Circadian regulation in human white adipose tissue revealed by transcriptome and metabolic network analysis

Christou, Skevoulla, Wehrens, Sophie M T, Isherwood, Cheryl, Moller-Levet, Carla S, Wu, Huihai, Revell, Victoria L, Bucca, Giselda, Skene, Debra J, Laing, Emma E, Archer, Simon N and Johnston, Jonathan D (2019) Circadian regulation in human white adipose tissue revealed by transcriptome and metabolic network analysis Scientific Reports.

[img]
Preview
Text
Circadian regulation in human white adipose tissue revealed by transcriptome and metabolic network analysis.pdf - Accepted version Manuscript
Available under License Creative Commons Attribution.

Download (986kB) | Preview
[img] Archive (All files, separate)
All files, separate.zip - Supplemental Material
Available under License Creative Commons Attribution.

Download (1MB)

Abstract

Studying circadian rhythms in most human tissues is hampered by difficulty in collecting serial samples. Here we reveal circadian rhythms in the transcriptome and metabolic pathways of human white adipose tissue. Subcutaneous adipose tissue was taken from seven healthy males under highly controlled ‘constant routine’ conditions. Five biopsies per participant were taken at six-hourly intervals for microarray analysis and in silico integrative metabolic modelling. We identified 837 transcripts exhibiting circadian expression profiles (2% of 41619 transcript targeting probes on the array), with clear separation of transcripts peaking in the morning (258 probes) and evening (579 probes). There was only partial overlap of our rhythmic transcripts with published animal adipose and human blood transcriptome data. Morning-peaking transcripts associated with regulation of gene expression, nitrogen compound metabolism, and nucleic acid biology; evening-peaking transcripts associated with organic acid metabolism, cofactor metabolism and redox activity. In silico pathway analysis further indicated circadian regulation of lipid and nucleic acid metabolism; it also predicted circadian variation in key metabolic pathways such as the citric acid cycle and branched chain amino acid degradation. In summary, in vivo circadian rhythms exist in multiple adipose metabolic pathways, including those involved in lipid metabolism, and core aspects of cellular biochemistry.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Christou, Skevoullas.christou@surrey.ac.uk
Wehrens, Sophie M TSophie.Wehrens@surrey.ac.uk
Isherwood, Cherylc.m.isherwood@surrey.ac.uk
Moller-Levet, Carla SC.Moller-Levet@surrey.ac.uk
Wu, HuihaiH.Wu@surrey.ac.uk
Revell, Victoria LV.Revell@surrey.ac.uk
Bucca, Giselda
Skene, Debra JD.Skene@surrey.ac.uk
Laing, Emma EE.Laing@surrey.ac.uk
Archer, Simon NSimon.Archer@surrey.ac.uk
Johnston, Jonathan DJ.Johnston@surrey.ac.uk
Date : 2019
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
Copyright Disclaimer : © The authors 2018. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Related URLs :
Depositing User : Clive Harris
Date Deposited : 31 Jan 2019 11:46
Last Modified : 31 Jan 2019 11:46
URI: http://epubs.surrey.ac.uk/id/eprint/850332

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800