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Characterization of a colistin-resistant Avian Pathogenic Escherichia coli ST69 isolate recovered from a broiler chicken in Germany

Hornsey, Michael, Betts, Jonathan W., Mehat, Jai W., Wareham, David W., van Vliet, Arnoud H. M., Woodward, Martin J. and La Ragione, Roberto M. (2019) Characterization of a colistin-resistant Avian Pathogenic Escherichia coli ST69 isolate recovered from a broiler chicken in Germany Journal of Medical Microbiology, 68 (1). pp. 111-114.

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Abstract

In recent years, several plasmids harbouring genes encoding phosphoethanolamine transferases conferring colistin resistance have been described in multiple Enterobacteriaceae species. Avian Pathogenic E. coli (APEC) causes colibacillosis and is responsible for a considerable proportion of the disease burden in commercial poultry flocks, and may be linked to zoonotic infections in humans. Here, we describe the genotypic and phenotypic characteristics of a multidrug-resistant APEC ST69 isolate (APECA2), recovered in 2016 from a diseased broiler at post-mortem examination in Germany. The isolate was resistant to several antibiotics of human and veterinary importance, including colistin. The mcr-1 gene was detected on a mobile genetic element located on an IncHI2/ST4 plasmid, which was characterized using long-read Nanopore and short-read Illumina sequencing of purified plasmid. Isolate APECA2 displayed resistance to chicken serum and harbours numerous virulence genes. This study highlights the public health importance of enhanced antimicrobial resistance surveillance and strict antimicrobial stewardship in human and veterinary healthcare.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Hornsey, Michaelmike.hornsey@surrey.ac.uk
Betts, Jonathan W.jono.betts@surrey.ac.uk
Mehat, Jai W.jw.mehat@surrey.ac.uk
Wareham, David W.
van Vliet, Arnoud H. M.a.vanvliet@surrey.ac.uk
Woodward, Martin J.
La Ragione, Roberto M.R.Laragione@surrey.ac.uk
Date : 1 January 2019
DOI : 10.1099/jmm.0.000882
Copyright Disclaimer : © 2019 The Authors. Published by the Microbiology Society
Uncontrolled Keywords : APEC; Avian Pathogenic Escherichia coli; Resistance; Germany; MCR-1
Additional Information : The GenBank accession number for the blaTEM-135 sequence of APECA2 is KY202655. The GenBank accession number for the plasmid, pJMA2 sequence of APECA2 is MH208235.
Depositing User : Clive Harris
Date Deposited : 31 Jan 2019 08:27
Last Modified : 31 Jan 2019 08:50
URI: http://epubs.surrey.ac.uk/id/eprint/850328

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