University of Surrey

Test tubes in the lab Research in the ATI Dance Research

A comparison of the in vitro permeation of niacinamide in mammalian skin and in the Parallel Artificial Membrane Permeation Assay (PAMPA) model

Zhang, Yanling, Lane, Majella E., Hadgraft, Jonathan, Heinrich, Michael, Chen, Tao, Lian, Guoping and Sinko, Balint (2019) A comparison of the in vitro permeation of niacinamide in mammalian skin and in the Parallel Artificial Membrane Permeation Assay (PAMPA) model International Journal of Pharmaceutics, 556. pp. 142-149.

[img] Text
A comparison of the in vitro permeation of niacinamide in mammalian skin and in the Parallel Artificial Membrane Permeation Assay (PAMPA) model.docx - Accepted version Manuscript

Download (274kB)

Abstract

The in vitro skin penetration of pharmaceutical or cosmetic ingredients is usually assessed in human or animal tissue. However, there are ethical and practical difficulties associated with sourcing these materials; variability between donors may also be problematic when interpreting experimental data. Hence, there has been much interest in identifying a robust and high throughput model to study skin permeation that would generate more reproducible results. Here we investigate the permeability of a model active, niacinamide (NIA), in (i) conventional vertical Franz diffusion cells with excised human skin or porcine skin and (ii) a recently developed Parallel Artificial Membrane Permeation Assay (PAMPA) model. Both finite and infinite dose conditions were evaluated in both models using a series of simple NIA solutions and one commercial preparation. The Franz diffusion cell studies were run over 24 h while PAMPA experiments were conducted for 2.5 h. A linear correlation between both models was observed for the cumulative amount of NIA permeated in tested models under finite dose conditions. The corresponding correlation coefficients (r²) were 0.88 for porcine skin and 0.71 for human skin. These results confirm the potential of the PAMPA model as a useful screening tool for topical formulations. Future studies will build on these findings and expand further the range of actives investigated.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemical and Process Engineering
Authors :
NameEmailORCID
Zhang, Yanling
Lane, Majella E.
Hadgraft, Jonathan
Heinrich, Michael
Chen, TaoT.Chen@surrey.ac.uk
Lian, Guopingg.lian@surrey.ac.uk
Sinko, Balint
Date : 10 February 2019
DOI : 10.1016/j.ijpharm.2018.11.065
Copyright Disclaimer : © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Skin; Permeation; Porcine; Human; PAMPA; Niacinamide
Depositing User : Clive Harris
Date Deposited : 05 Dec 2018 13:24
Last Modified : 09 Sep 2019 08:37
URI: http://epubs.surrey.ac.uk/id/eprint/849984

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800