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Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer

Chew, Ming Tsuey, Nisbet, Andrew, Suzuki, Masao, Matsufuji, Naruhiro, Murakami, Takeshi, Jones, Bleddyn and Bradley, David A. (2018) Potential lethal damage repair in glioblastoma cells irradiated with ion beams of various types and levels of linear energy transfer Journal of Radiation Research, rry081.

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Abstract

Glioblastoma (GBM), a Grade IV brain tumour, is a well-known radioresistant cancer. To investigate one of the causes of radioresistance, we studied the capacity for potential lethal damage repair (PLDR) of three altered strains of GBM: T98G, U87 and LN18, irradiated with various ions and various levels of linear energy transfer (LET). The GBM cells were exposed to 12C and 28Si ion beams with LETs of 55, 100 and 200 keV/μm, and with X-ray beams of 1.7 keV/μm. Mono-energetic 12C ions and 28Si ions were generated by the Heavy Ion Medical Accelerator at the National Institute of Radiological Science, Chiba, Japan. Clonogenic assays were used to determine cell inactivation. The ability of the cells to repair potential lethal damage was demonstrated by allowing one identical set of irradiated cells to repair for 24 h before subplating. The results show there is definite PLDR with X-rays, some evidence of PLDR at 55 keV/μm, and minimal PLDR at 100 keV/μm. There is no observable PLDR at 200 keV/μm. This is the first study, to the authors’ knowledge, demonstrating the capability of GBM cells to repair potential lethal damage following charged ion irradiations. It is concluded that a GBM’s PLDR is dependent on LET, dose and GBM strain; and the more radioresistant the cell strain, the greater the PLDR.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Physics
Authors :
NameEmailORCID
Chew, Ming Tsuey
Nisbet, AndrewA.Nisbet@surrey.ac.uk
Suzuki, Masao
Matsufuji, Naruhiro
Murakami, Takeshi
Jones, Bleddyn
Bradley, David A.
Date : 17 November 2018
Funders : FP7
DOI : 10.1093/jrr/rry081
Copyright Disclaimer : © The Author(s) 2018. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial reuse, please contact journals.permissions@oup.com
Depositing User : Melanie Hughes
Date Deposited : 20 Nov 2018 15:02
Last Modified : 11 Dec 2018 11:24
URI: http://epubs.surrey.ac.uk/id/eprint/849905

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