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A novel Biomarker in epithelial ovarian cancer

McGrath, Sophie Elena, Annels, Nicola, Madhuri, Thumuluru K, Taylor, Anil, Butler-Manuel, Simon A., Morgan, Richard, Pandha, Hardev and Michael, Agnieszka (2018) A novel Biomarker in epithelial ovarian cancer BMC Cancer, 18, 943.

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Abstract

BACKGROUND: Epithelial ovarian cancer is a common malignancy, with no clinically approved diagnostic biomarker. Engrailed-2 (EN2) is a homeodomain-containing transcription factor, essential during embryological neural development, which is dysregulated in several cancer types. We evaluated the expression of EN2 in Epithelial ovarian cancer, and reviewed its role as a biomarker. METHODS We evaluated 8 Epithelial ovarian cancer cell lines, along with >100 surgical specimens from the Royal Surrey County Hospital (2009-2014). In total, 108 tumours and 5 normal tissue specimens were collected. En2 mRNA was evaluated by semi-quantitative RT-PCR. Histological sub-type, and platinum-sensitive/-resistant status were compared. Protein expression was assessed in cell lines (immunofluorescence), and in >150 tumours (immunohistochemistry). RESULTS En2 mRNA expression was elevated in serous ovarian tumours compared with normal ovary (p<0.001), particularly in high-grade serous ovarian cancer (p<0.0001) and in platinum-resistant tumours (p=0.0232). Median Overall Survival and Progression-free Survival were reduced with high En2 expression (OS=28 vs 42 months, p=0.0329; PFS=8 vs 27 months; p=0.0004). Positive cytoplasmic EN2 staining was demonstrated in 78% of Epithelial ovarian cancers, with absence in normal ovary. EN2 positive high-grade serous ovarian cancer patients had a shorter PFS (10 vs 17.5 months; p=0.0103). CONCLUSION The EN2 transcription factor is a novel ovarian cancer biomarker. It demonstrates prognostic value, correlating with worse Overall Survival and Progression-free Survival. It is hoped that further work will validate its use as a biomarker, and provide insight into the role of EN2 in the development, progression and spread of ovarian cancer.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
McGrath, Sophie Elena
Annels, Nicola
Madhuri, Thumuluru K
Taylor, Anil
Butler-Manuel, Simon A.
Morgan, RichardR.Morgan@surrey.ac.uk
Pandha, HardevH.Pandha@surrey.ac.uk
Michael, AgnieszkaA.Michael@surrey.ac.uk
Date : 3 October 2018
DOI : 10.1186/s12885-018-4816-5
Copyright Disclaimer : © The Author(s). 2018 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Uncontrolled Keywords : Epithelial ovarian cancer; biomarker; engrailed-2; serous; platinum-resistant
Depositing User : Melanie Hughes
Date Deposited : 13 Nov 2018 11:57
Last Modified : 19 Mar 2019 10:22
URI: http://epubs.surrey.ac.uk/id/eprint/849864

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