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Reviving phage therapy for the treatment of Cholera

Bhandare, Sudhakar, Colom, Joan, Baig, Abiyad, Ritchie, Jennifer M, Bokhari, Habib, Shah, Muhammad A, Sarkar, Banwarilal L, Su, Jingliang, Wren, Brendan, Barrow, Paul A and Atterbury, Robert (2018) Reviving phage therapy for the treatment of Cholera The Journal of Infectious Diseases, 219 (5). pp. 786-794.

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Cholera remains a major risk in developing countries, particularly after natural or man-made disasters. Vibrio cholerae El Tor is the most important cause of these outbreaks, and is becoming increasingly resistant to antibiotics, so alternative therapies are urgently needed. In this study, a single bacteriophage, Phi_1, was used to control cholera prophylactically and therapeutically in an infant rabbit model. In both cases, phage-treated animals showed no clinical signs of disease, compared with 69% of untreated control animals. Bacterial counts in the intestines of phage-treated animals were reduced by up to 4 log10 colony-forming units/g. There was evidence of phage multiplication only in animals that received a V. cholerae challenge. No phage-resistant bacterial mutants were isolated from the animals, despite extensive searching. This is the first evidence that a single phage could be effective in the treatment of cholera, without detectable levels of resistance. Clinical trials in human patients should be considered.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Bhandare, Sudhakar
Colom, Joan
Baig, Abiyad
Ritchie, Jennifer
Bokhari, Habib
Shah, Muhammad A
Sarkar, Banwarilal L
Su, Jingliang
Wren, Brendan
Barrow, Paul A
Atterbury, Robert
Date : 3 November 2018
Funders : UK-India Education and Research Initiative
DOI : 10.1093/infdis/jiy563
Copyright Disclaimer : © The Author(s) 2018. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (
Uncontrolled Keywords : Bacteriophage therapy; Cholera; Phage; Infant rabbit; Prophylaxis; Vibrio cholerae
Depositing User : Clive Harris
Date Deposited : 07 Nov 2018 12:01
Last Modified : 04 Nov 2019 02:08

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