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Application of Various Normalisation Methods for Microscale Analysis of Tissues Using Direct Analyte Probed Nano-extraction (DAPNe)

De Jesus, Janella, Bunch, Josephine, Verbeck, Guido, Webb, Roger, Costa, Catia, Goodwin, Richard and Bailey, Melanie (2018) Application of Various Normalisation Methods for Microscale Analysis of Tissues Using Direct Analyte Probed Nano-extraction (DAPNe) Analytical Chemistry, 90 (20). pp. 12094-12100.

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Abstract

Direct analyte-probed nano-extraction (DAPNe) is a method of extracting material from a microscale region of a sample and provides the opportunity for detailed mass spectrometry analysis of extracted analytes from a small area. The technique has been shown to provide enhanced sensitivity compared with bulk analysis by selectively removing analytes from their matrix and has been applied for selective analysis of single cells and even single organelles. However, the quantitative capabilities of the technique are yet to be fully evaluated. In this study, various normalisation techniques were investigated in order to improve the quantitative capabilities of the technique. Two methods of internal standard incorporation were applied to test substrates, which were designed to replicate biological sample matrices. Additionally, normalisation to the extraction spot area and matrix compounds were investigated for suitability in situations when an internal standard is not available. The variability observed can be significantly reduced by using a sprayed internal standard, and in some cases, by normalising to the extracted area.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemistry
Authors :
NameEmailORCID
De Jesus, Janellaj.dejesus@surrey.ac.uk
Bunch, Josephine
Verbeck, Guido
Webb, RogerR.Webb@surrey.ac.uk
Costa, Catiac.d.costa@surrey.ac.uk
Goodwin, Richard
Bailey, MelanieM.Bailey@surrey.ac.uk
Date : 27 September 2018
Funders : EPSRC
DOI : 10.1021/acs.analchem.8b03016
Copyright Disclaimer : ACS AuthorChoice - This is an open access article published under a Creative Commons Attribution (CC-BY) License, which permits unrestricted use, distribution and reproduction in any medium, provided the author and source are cited.
Depositing User : Melanie Hughes
Date Deposited : 25 Sep 2018 11:48
Last Modified : 11 Dec 2018 11:24
URI: http://epubs.surrey.ac.uk/id/eprint/849428

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