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Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine

Sui, Tan, Dluhoš, Jiří, Li, Tao, Zeng, Kaiyang, Cernescu, Adrian, Landini, Gabriel and Korsunsky, Alexander (2018) Structure-Function Correlative Microscopy of Peritubular and Intertubular Dentine Materials, 11 (9).

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Abstract

Peritubular dentine (PTD) and intertubular dentine (ITD) were investigated by 3D correlative Focused Ion Beam (FIB)-Scanning Electron Microscopy (SEM)-Energy Dispersive Spectroscopy (EDS) tomography, tapping mode Atomic Force Microscopy (AFM) and scattering-type Scanning Near-Field Optical Microscopy (s-SNOM) mapping. The brighter appearance of PTD in 3D SEM-Backscattered-Electron (BSE) imaging mode and the corresponding higher grey value indicate a greater mineral concentration in PTD (~160) compared to ITD (~152). However, the 3D FIB-SEM-EDS reconstruction and high resolution, quantitative 2D map of the Ca/P ratio (~1.8) fail to distinguish between PTD and ITD. This has been further confirmed using nanoscale 2D AFM map, which clearly visualised biopolymers and hydroxyapatite (HAp) crystallites with larger mean crystallite size in ITD (32 ± 8 nm) than that in PTD (22 ± 3 nm). Correlative microscopy reveals that the principal difference between PTD and ITD arises primarily from the nanoscale packing density of the crystallites bonded together by thin biopolymer, with moderate contribution from the chemical composition difference. The structural difference results in the mechanical properties variation that is described by the parabolic stiffness-volume fraction correlation function introduced here. The obtained results benefit a microstructure-based mechano-chemical model to simulate the chemical etching process that can occur in human dental caries and some of its treatments.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
NameEmailORCID
Sui, Tant.sui@surrey.ac.uk
Dluhoš, Jiří
Li, Tao
Zeng, Kaiyang
Cernescu, Adrian
Landini, Gabriel
Korsunsky, Alexander
Date : 21 August 2018
Funders : EPSRC, RCUK
DOI : 10.3390/ma11091493
Copyright Disclaimer : © 2018 by the authors.
Projects : Tackling human dental caries by multi-modal correlative microscopy and multi-physics modelling
Uncontrolled Keywords : Peritubular dentine (PTD); Intertubular dentine (ITD); FIB-SEM-EDS tomography; Tapping mode AFM; s-SNOM; Parabolic stiffness-volume fraction correlation function
Depositing User : Diane Maxfield
Date Deposited : 21 Sep 2018 09:57
Last Modified : 11 Dec 2018 11:24
URI: http://epubs.surrey.ac.uk/id/eprint/849390

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