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Qualitative differences in capsidless L-particles released as a by-product of bovine herpesvirus 1 and herpes simplex virus 1 infections

Russell, Tiffany, Bleasdale, Ben, Hollinshead, Michael and Elliott, Gillian (2018) Qualitative differences in capsidless L-particles released as a by-product of bovine herpesvirus 1 and herpes simplex virus 1 infections Journal of Virology, 92 (22), e01259-18.

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Abstract

Despite differences in the pathogenesis and host range of alphaherpesviruses, many stages of their morphogenesis are thought to be conserved. Here, an ultrastructural study of bovine herpesvirus 1 (BoHV-1) envelopment revealed similar profiles to those previously found for HSV-1, with BoHV-1 capsids associating with endocytic tubules. Consistent with the similarity of their genomes and envelopment strategies, the proteomic composition of BoHV-1 and HSV-1 virions was also comparable. However, BoHV-1 morphogenesis exhibited a diversity in envelopment events. First, heterogeneous primary envelopment profiles were readily detectable at the inner nuclear membrane of BoHV-1 infected cells. Second, the BoHV-1 progeny comprised not just full virions, but also an abundance of capsidless, non-infectious light (L)-particles that were released from the infected cell in similar numbers to virions, and in the absence of DNA replication. Proteomic analysis of BoHV-1 L- particles and the much less abundant HSV-1 L-particles revealed that they contained the same complement of envelope proteins as virions but showed variations in tegument content. In the case of HSV-1, the UL46 tegument protein was reproducibly found to be more than 6-fold enriched in HSV-1 L-particles. More strikingly, the tegument proteins UL36, UL37, UL21 and UL16 were depleted in BoHV-1 but not HSV-1 L-particles. We propose that these combined differences reflect the presence of a truly segregated “inner” and “outer” tegument in BoHV-1, making it a critical system for studying the structure and process of tegumentation and envelopment.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Russell, Tiffanyt.russell@surrey.ac.uk
Bleasdale, Ben
Hollinshead, Michael
Elliott, Gilliang.elliott@surrey.ac.uk
Date : 5 September 2018
Funders : Medical Research Council (MRC)
DOI : 10.1128/JVI.01259-18
Copyright Disclaimer : Copyright © 2018 Russell et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
Related URLs :
Depositing User : Clive Harris
Date Deposited : 29 Aug 2018 00:39
Last Modified : 11 Dec 2018 11:24
URI: http://epubs.surrey.ac.uk/id/eprint/849144

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