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Protein Synthesis during Sleep Consolidates Cortical Plasticity In Vivo

Seibt, Julie, Dumoulin, Michelle C, Aton, Sara J, Coleman, Tammi, Watson, Adam, Naidoo, Nirinjini and Frank, Marcos G (2012) Protein Synthesis during Sleep Consolidates Cortical Plasticity In Vivo Current Biology, 22. pp. 676-682.

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Abstract

Sleep consolidates experience-dependent brain plasticity, but the precise cellular mechanisms mediating this process are unknown [1]. De novo cortical protein synthesis is one possible mechanism. In support of this hypothesis, sleep is associated with increased brain protein synthesis [2, 3] and transcription of messenger RNAs (mRNAs) involved in protein synthesis regulation [4, 5]. Protein synthesis in turn is critical for memory consolidation and persistent forms of plasticity in vitro and in vivo [6, 7]. However, it is unknown whether cortical protein synthesis in sleep serves similar functions. We investigated the role of protein synthesis in the sleep-dependent consolidation of a classic form of cortical plasticity in vivo (ocular dominance plasticity, ODP; [8, 9]) in the cat visual cortex. We show that intracortical inhibition of mammalian target of rapamycin (mTOR)-dependent protein synthesis during sleep abolishes consolidation but has no effect on plasticity induced during wakefulness. Sleep also promotes phosphorylation of protein synthesis regulators (i.e., 4E-BP1 and eEF2) and the translation (but not transcription) of key plasticity related mRNAs (ARC and BDNF). These findings show that sleep promotes cortical mRNA translation. Interruption of this process has functional consequences, because it abolishes the consolidation of experience in the cortex.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Seibt, Juliej.seibt@surrey.ac.uk
Dumoulin, Michelle C
Aton, Sara J
Coleman, Tammi
Watson, Adam
Naidoo, Nirinjini
Frank, Marcos G
Date : 1 March 2012
DOI : 10.1016/j.cub.2012.02.016
Depositing User : Melanie Hughes
Date Deposited : 28 Aug 2018 12:43
Last Modified : 28 Aug 2018 12:43
URI: http://epubs.surrey.ac.uk/id/eprint/849124

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