University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression

Schanz, A., Red-Horse, K., Hess, A.P., Baston-Büst, D.M., Heiss, C. and Krüssel, J.S. (2014) Oxygen regulates human cytotrophoblast migration by controlling chemokine and receptor expression Placenta, 35 (12). pp. 1089-1094.

Full text not available from this repository.

Abstract

Introduction

Placental development involves the variation of oxygen supply due to vascular changes and cytotrophoblast invasion. Chemokines and their receptors play an important role during placental formation. Herein, the analysis of the chemokine/receptor pair CXCL12/CXCR4 and further chemokine receptors, such as CCR1, CCR7 and CXCR6 expression in human cytotrophoblasts was conducted.

Methods

Human cytotrophoblasts were examined directly after isolation or after incubation with different oxygen tensions and a chemical HIF-stimulator for 12 h with realtime PCR, immunoblot, immunohistochemistry. Conditioned media of placental villi, decidua, and endothelial cells was used for ELISA analysis of CXL12. Cytotrophoblast migration assays were conducted applying conditioned media of endothelial cells, a CXCL12 gradient, and different oxygen level. Endometrial and decidual tissue was stained for CXCL12 expression.

Results

An upregulation of CXCL12, CXCR4, CCR1, CCR7 and CXCR6 was observed after cytotrophoblast differentiation. Low oxygen supply upregulated CXCR4, CCR7 and CXCR6, but downregulated CXCL12 and CCR1. In contrast to the HIF associated upregulation of the aforementioned proteins, downregulation of CXCL12 and CCR1 seemed to be HIF independent. Cytotrophoblast migration was stimulated by low oxygen, the application of a CXCL12 gradient and endothelial cell conditioned media. CXCL12 was detected in endometrial vessels, glands and conditioned media of placental and decidual tissue, but not decidual vessels.

Discussion/conclusion

Taken together, oxygen supply and cytotrophoblast differentiation seem to be regulators of chemokine and receptor expression and function in human cytotrophoblasts. Therefore, this system seems to be involved in placental development, directed cytotrophoblast migration in the decidual compartment and a subsequent sufficient supply of the growing fetus.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Clinical and Experimental Medicine
Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Schanz, A.
Red-Horse, K.
Hess, A.P.
Baston-Büst, D.M.
Heiss, C.c.heiss@surrey.ac.uk
Krüssel, J.S.
Date : 26 September 2014
Identification Number : 10.1016/j.placenta.2014.09.012
Copyright Disclaimer : Copyright © 2014 Elsevier Ltd. All rights reserved.
Uncontrolled Keywords : Chemokine; Chemokine receptor; Hypoxia; Placenta; Cytotrophoblast
Depositing User : Diane Maxfield
Date Deposited : 20 Aug 2018 10:30
Last Modified : 20 Aug 2018 10:30
URI: http://epubs.surrey.ac.uk/id/eprint/849045

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800