University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Uptake and metabolism of (−)-epicatechin in endothelial cells

Rodriguez-Mateos, Ana, Toro-Funes, Natalia, Cifuentes-Gomez, Tania, Cortese-Krott, Miriam, Heiss, Christian and Spencer, Jeremy P.E. (2014) Uptake and metabolism of (−)-epicatechin in endothelial cells ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 559. pp. 17-23.

Full text not available from this repository.

Abstract

Accumulating evidence suggest that diets rich in cocoa flavanols may have beneficial effects on cardiovascular health. The major cocoa flavanol monomer, (−)-epicatechin (EC), is readily absorbed and circulates primarily as glucuronidated, sulfated, and O-methylated metabolites in human plasma. However, cellular metabolism, for example in endothelial cells, is less well defined. In the present study we detail the uptake and cellular metabolism of EC and its major in vivo metabolites, (−)-epicatechin-3′-β-d-glucuronide (E3G), (−)-epicatechin-3′-sulfate (E3S), 3′-O-methyl-(−)-epicatechin-5-sulfate (3ME5S), and 3′-O-methyl-(−)-epicatechin-7-sulfate (3ME7S) in human endothelial (HUVEC), liver (HepG2) and intestinal epithelial cells (Caco-2 monolayer). Our results indicate that EC associates with HUVECs, leading to its intracellular metabolism to 3ME7G and 3ME7S. In contrast, none of the metabolites were taken up by the cells. The metabolic rate and pattern of metabolism in HUVECs was similar to that observed in HepG2 cells, whilst in Caco-2 cells EC was metabolized to E3G, 3ME5G, 3ME7G, 4ME5G, 4ME7G and 3ME7S. Our data support the notion that endothelial cells may contribute significantly to EC metabolism. However, major human circulating metabolites are not accounted for in these model systems underscoring that caution should be taken when drawing conclusions on in vivo flavanol metabolism from in vitro experiments.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Clinical and Experimental Medicine
Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Rodriguez-Mateos, Ana
Toro-Funes, Natalia
Cifuentes-Gomez, Tania
Cortese-Krott, Miriam
Heiss, Christianc.heiss@surrey.ac.uk
Spencer, Jeremy P.E.
Date : 1 October 2014
Identification Number : 10.1016/j.abb.2014.03.014
Copyright Disclaimer : Copyright © 2014 Elsevier Inc. All rights reserved.
Uncontrolled Keywords : (-)-Epicatechin; Uptake; Phase II metabolism; HUVEC; Endothelial cells; HepG2; Caco-2 monolayer
Depositing User : Diane Maxfield
Date Deposited : 17 Aug 2018 15:33
Last Modified : 17 Aug 2018 15:33
URI: http://epubs.surrey.ac.uk/id/eprint/849035

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800