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A systematic approach to assess locoregional differences in angiogenesis

Driesen, T., Schuler, D., Schmetter, R., Heiss, C., Kelm, M., Fischer, J. W. and Freudenberger, T. (2015) A systematic approach to assess locoregional differences in angiogenesis Histochemistry and Cell Biology, 145 (2). pp. 213-225.

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Skeletal muscle tissue differs with regard to the abundance of glycolytic and oxidative fiber types. In this context, capillary density has been described to be higher in muscle tissue with more oxidative metabolism as compared to that one with more glycolytic metabolism, and the highest abundance of capillaries has been found in boneward-oriented moieties of skeletal muscle tissue. Importantly, capillary formation is often analyzed as a measure for angiogenesis, a process that describes neo-vessel formation emanating from preexisting vessels, occurring, i.e., after arterial occlusion. However, a standardized way for investigation of calf muscle capillarization after surgically induced unilateral hind limb ischemia in mice, especially considering these locoregional differences, has not been provided so far. In this manuscript, a novel, methodical approach for reliable analysis of capillary density was established using anatomic–morphological reference points, and a software-assisted way of capillary density analysis is described. Thus, the systematic approach provided conscientiously considers intra-layer differences in capillary formation and therefore guarantees for a robust, standardized analysis of capillary density as a measure for angiogenesis. The significance of the methodology is further supported by the observation that capillary density in the calf muscle layers analyzed negatively correlates with distal lower limb perfusion measured in vivo.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
Driesen, T.
Schuler, D.
Schmetter, R.
Heiss, C.
Kelm, M.
Fischer, J. W.
Freudenberger, T.
Date : 2 November 2015
DOI : 10.1007/s00418-015-1379-2
Uncontrolled Keywords : Hind limb ischemia; Capillary density; Angiogenesis; Immunohistochemistry
Depositing User : Melanie Hughes
Date Deposited : 16 Aug 2018 09:45
Last Modified : 16 Aug 2018 09:45

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