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hCG stimulates angiogenic signals in lymphatic endothelial and circulating angiogenic cells

Schanz, Andrea, Lukosz, Margarete, Hess, Alexandra P, Baston-Büst, Dunja M, Krüssel, Jan S and Heiss, Christian (2015) hCG stimulates angiogenic signals in lymphatic endothelial and circulating angiogenic cells Journal of Reproductive Immunology, 110. pp. 102-108.

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Abstract

Human chorionic gonadotropin (hCG) has long been associated with the initiation and maintenance of pregnancy, where angiogenesis plays an important role. However, the function of hCG in angiogenesis and the recruitment of vascular active cells are not fully understood. In this study, the role of hCG and its receptor in circulating angiogenic and human endothelial cells, including lymphatic, uterine microvascular, and umbilical vein endothelial cells, was examined. Immunohistochemistry and immunoblot analysis were used to detect LH/hCG receptor expression and the expression of hCG-induced angiogenic molecules. HIF-1α was determined via ELISA and downstream molecules, such as CXCL12 and CXCR4, via real-time PCR. Chemotaxis was analyzed using Boyden chambers. Our results show that the LH/hCG receptor was present in all tested cells. Furthermore, hCG was able to stimulate LH/hCG-receptor-specific migration in a dose-dependent fashion and induce key angiogenic molecules, including HIF-1α, CXCL12, and CXCR4. In conclusion, our findings underscore the importance of hCG as one of the first angiogenic molecules produced by the conceptus. hCG itself alters endothelial motility, recruitment, and expression of pro-angiogenic molecules and may therefore play an important role in vascular adaption during implantation and early placental formation.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Schanz, Andrea
Lukosz, Margarete
Hess, Alexandra P
Baston-Büst, Dunja M
Krüssel, Jan S
Heiss, Christianc.heiss@surrey.ac.uk
Date : 24 February 2015
DOI : 10.1016/j.jri.2015.01.011
Uncontrolled Keywords : Angiogenesis; Human chorionic gonadotropin; hCG; Lymphatic endothelial cells; Circulating angiogenic cells
Depositing User : Melanie Hughes
Date Deposited : 15 Aug 2018 14:15
Last Modified : 15 Aug 2018 14:15
URI: http://epubs.surrey.ac.uk/id/eprint/848953

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