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Characterization of the Non-Invasive Assessment of the Cutaneous Microcirculation by Laser Doppler Perfusion Scanner

KEYMEL, STEFANIE, SICHWARDT, JULIA, BALZER, JAN, STEGEMANN, EMILIA, RASSAF, TIENUSH, KLEINBONGARD, PETRA, KELM, MALTE, Heiss, Christian and LAUER, Thomas (2010) Characterization of the Non-Invasive Assessment of the Cutaneous Microcirculation by Laser Doppler Perfusion Scanner Microcirculation, 17. pp358-366.

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Abstract

Objective:  Microcirculatory dysfunction contributes to morbidity and mortality in vascular diseases. Here, we aimed at establishing a sensitive and valid method to measure microvascular reactivity during post‐occlusive reactive hyperemia (PORH) using scanning laser Doppler perfusion imaging (LDPI) of the forearm. Methods:  In a first series, LDPI was methodologically evaluated on the volar forearm of healthy volunteers (n = 10) before and after one to five minutes of upper arm occlusion. In a second series, readings were performed in 20 healthy subjects and 20 patients with coronary artery disease (CAD). Results:  Three minutes of forearm occlusion were sufficient to induce maximal vasodilation during PORH as indicated by maximal increase in perfusion unit (PU) amplitude that did not further increase after five‐minute occlusion. Five‐minute occlusion led to a significant prolongation of PORH with greater area under curve (AUC) suggesting longer lasting vasodilation of microvessels. The five‐minute occlusion was associated with lower variability as compared with three minutes (intraindividual variability: 9–17% vs. 12–21%; interindividual variability: 13–24% vs. 14–26%). CAD patients exhibited significantly reduced amplitude (105 ± 49 vs. 164 ± 35 PU; p < 0.001), ratio (4.7 ± 1.8 vs. 7.1 ± 1.8; p < 0.001), and AUC (1656 ± 1070 vs. 2723 ± 864 PU × minutes; p = 0.001). Conclusion:  Scanning LDPI is a feasible and reproducible method for non‐invasive assessment of the cutaneous microcirculatory response during PORH.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Clinical and Experimental Medicine
Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
KEYMEL, STEFANIE
SICHWARDT, JULIA
BALZER, JAN
STEGEMANN, EMILIA
RASSAF, TIENUSH
KLEINBONGARD, PETRA
KELM, MALTE
Heiss, Christianc.heiss@surrey.ac.uk
LAUER, Thomas
Date : 2 July 2010
DOI : 10.1111/j.1549-8719.2010.00037.x
Copyright Disclaimer : © 2010 John Wiley & Sons Ltd
Uncontrolled Keywords : Laser Doppler perfusion imaging; Coronary artery disease; Microcirculation
Depositing User : Diane Maxfield
Date Deposited : 14 Aug 2018 15:09
Last Modified : 14 Aug 2018 15:09
URI: http://epubs.surrey.ac.uk/id/eprint/848934

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