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Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor.

Bucca, Giselda, Pothi, Radhika, Hesketh, Andrew, Moller-Levet, Carla, Hodgson, David A, Laing, Emma, Stewart, Graham and Smith, Colin (2018) Translational control plays an important role in the adaptive heat-shock response of Streptomyces coelicolor. Nucleic Acids Research, 46 (11). pp. 5692-5703.

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Abstract

Stress-induced adaptations requiremultiple levels of regulation in all organisms to repair cellular damage. In the present study we evaluated the genome-wide transcriptional and translational changes following heat stress exposure in the soil-dwelling model actinomycete bacterium, Streptomyces coelicolor. The combined analysis revealed an unprecedented level of translational control of gene expression, deduced through polysome profiling, in addition to transcriptional changes. Our data show little correlation between the transcriptome and ‘translatome’; while an obvious downward trend in genome wide transcription was observed, polysome associated transcripts following heat-shock showed an opposite upward trend. A handful of key protein players, including the major molecular chaperones and proteases were highly induced at both the transcriptional and translational level following heat-shock, a phenomenon known as ‘potentiation’. Many other transcripts encoding cold-shock proteins, ABC-transporter systems, multiple transcription factors weremore highly polysome-associated following heat stress; interestingly, these protein families were not induced at the transcriptional level and therefore were not previously identified as part of the stress response. Thus, stress coping mechanisms at the level of gene expression in this bacterium go well beyond the induction of a relatively small number of molecular chaperones and proteases in order to ensure cellular survival at non-physiological temperatures.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Bucca, Giselda
Pothi, Radhika
Hesketh, Andrew
Moller-Levet, CarlaC.Moller-Levet@surrey.ac.uk
Hodgson, David A
Laing, EmmaE.Laing@surrey.ac.uk
Stewart, GrahamG.Stewart@surrey.ac.uk
Smith, Colin
Date : 9 May 2018
Funders : BBSRC
DOI : 10.1093/nar/gky335
Copyright Disclaimer : Copyright The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Depositing User : Melanie Hughes
Date Deposited : 14 Aug 2018 11:25
Last Modified : 11 Dec 2018 11:24
URI: http://epubs.surrey.ac.uk/id/eprint/848922

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