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The Genome Sequence of a SNP Type 3K strain of Mycobacterium leprae isolated from a 7th Century Hungarian case of Lepromatous Leprosy.

Mendum, Thomas, Taylor, G Michael, Donoghue, Helen D, Wu, Huihai, Szalontai, Csaba, Marcsik, Antónia, Molnár, Erika, Pálfi, György and Stewart, Graham (2018) The Genome Sequence of a SNP Type 3K strain of Mycobacterium leprae isolated from a 7th Century Hungarian case of Lepromatous Leprosy. International Journal of Osteoarchaeology, 28 (4). pp. 439-447.

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Abstract

We report on a Mycobacterium leprae genome isolated from the remains of an individual with lepromatous leprosy that were excavated from a 7th century Hungarian cemetery. We determined that the genome was from a SNP type 3K0 M. leprae strain, a lineage that diverged early from other M. leprae lineages. This is one of the earliest 3K0 M. leprae genomes to be sequenced to date. A number of novel SNPs as well as SNPs characteristic of the 3K0 lineage were confirmed by conventional PCR and Sanger sequencing. Recovery of accompanying human DNA from the burial was poor, particularly when compared to that of the pathogen. Modern 3K0 M. leprae strains have only been isolated from East Asia and the Pacific and so these findings require new scenarios to describe the origins and routes of dissemination of leprosy during antiquity that have resulted in the modern phylogeographical distribution of M. leprae.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Mendum, ThomasT.Mendum@surrey.ac.uk
Taylor, G Michael
Donoghue, Helen D
Wu, HuihaiH.Wu@surrey.ac.uk
Szalontai, Csaba
Marcsik, Antónia
Molnár, Erika
Pálfi, György
Stewart, GrahamG.Stewart@surrey.ac.uk
Date : 23 May 2018
DOI : 10.1002/oa.2673
Copyright Disclaimer : This is the peer reviewed version of the following article: Mendum TA, Taylor GM, Donoghue HD, et al. The genome sequence of an SNP type 3K strain of Mycobacterium leprae isolated from a seventh‐century Hungar- ian case of lepromatous leprosy. Int J Osteoarchaeol. 2018;28:439–447. https://doi.org/10.1002/oa.2673 which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/oa.2673. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Uncontrolled Keywords : Ancient DNA, Mycobacterium leprae, phylogeny, Branch 0, whole genome sequencing.
Depositing User : Melanie Hughes
Date Deposited : 14 Aug 2018 11:03
Last Modified : 11 May 2019 02:08
URI: http://epubs.surrey.ac.uk/id/eprint/848921

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