University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Nuclear exclusion of SMAD2/3 in granulosa cells is associated with cell proliferation and follicle activation in the mouse ovary.

Hardy, Kate, Mora, Jocelyn M, Dunlop, Carina, Carzaniga, Raffaella, Franks, Stephen and Fenwick, Mark A (2018) Nuclear exclusion of SMAD2/3 in granulosa cells is associated with cell proliferation and follicle activation in the mouse ovary. Journal of Cell Science, 131, jcs218123.

[img]
Preview
Text
__homes.surrey.ac.uk_home_.System_Desktop_jcs.218123.full.pdf - Accepted version Manuscript

Download (10MB) | Preview

Abstract

Maintenance and activation of the limited supply of primordial follicles in the ovary are important determinants of reproductive lifespan. Currently, the molecular programme that maintains the primordial phenotype and the early events associated with follicle activation are not well defined. Here we have systematically analysed these events using microscopy and detailed image analysis. Using the immature mouse ovary as a model, we demonstrate that the onset of granulosa cell (GC) proliferation results in increased packing density on the oocyte surface and consequent GC cuboidalisation. These events precede oocyte growth and nuclear translocation of FOXO3a, a transcription factor important in follicle activation. Immunolabelling of the TGF signalling mediators and transcription factors, SMAD2/3, revealed a striking expression pattern specific to GCs of small follicles. SMAD2/3 was expressed in the nuclei of primordial GCs but was mostly excluded in early growing follicles. In activated follicles, GC nuclei lacking SMAD2/3 generally expressed Ki67. These findings suggest that the first phenotypic changes during follicle activation are observed in GCs, and that TGF signalling is fundamental for regulating GC arrest and the onset of proliferation.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Mathematics
Authors :
NameEmailORCID
Hardy, Kate
Mora, Jocelyn M
Dunlop, Carinac.dunlop@surrey.ac.uk
Carzaniga, Raffaella
Franks, Stephen
Fenwick, Mark A
Date : 15 August 2018
Funders : Biotechnology and Biological Sciences Research Council (BBSRC), Medical Research Council (MRC)
DOI : 10.1242/jcs.218123
Copyright Disclaimer : © 2018. Published by The Company of Biologists Ltd http://www.biologists.com/user-licence-1-1/
Depositing User : Melanie Hughes
Date Deposited : 09 Aug 2018 09:59
Last Modified : 16 Aug 2019 02:08
URI: http://epubs.surrey.ac.uk/id/eprint/848881

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800