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Characterisation of the myeloid cell populations’ resident in the porcine palatine tonsil

Soldevila, Ferran, Edwards, Jane C., Graham, Simon P., Stevens, Lisa M., Crudgington, Bentley, Crooke, Helen R., Werling, Dirk and Steinbach, Falko (2018) Characterisation of the myeloid cell populations’ resident in the porcine palatine tonsil Frontiers in Immunology, 9, 1800. pp. 1-16.

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Abstract

The palatine tonsil is the portal of entry for food and air, and is continuously subjected to environmental challenges including pathogens which use the tonsil and pharynx as a primary site of replication. In pigs, this includes the viruses causing porcine respiratory and reproductive syndrome, and classical and African swine fever; diseases which have impacted the pig production industry globally. Despite the importance of tonsils in host defence, little is known regarding the phenotype of the myeloid cells resident in the porcine tonsil. Here, we have characterised five myeloid cell populations that align to orthologous populations defined in other mammalian species: a CD4+ plasmacytoid DC (pDC) defined by expression of the conserved markers E2.2 and IRF-7, a conventional dendritic cell (cDC1) population expressing CADM1highCD172alow and high levels of XCR1 able to activate allogeneic CD4 and CD8 T cells; a cDC2 population of CADM1dim cells expressing FLT3, IRF4 and CSF1R with an ability to activate allogeneic CD4 T cells; CD163+ macrophages (MƟs) defined by high levels of endocytosis and responsiveness to LPS and finally a CD14+ population likely derived from a myelo-monocytic lineage, which showed the highest levels of endocytosis, a capacity for activation of CD4+ memory cells, combined with lower relative expression of FLT3. Increased knowledge regarding the phenotypic and functional properties of myeloid cells resident in porcine tonsil, will enable these cells to be targeted for future vaccination strategies to current and emerging porcine viruses.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Soldevila, Ferran
Edwards, Jane C.
Graham, Simon P.s.graham@surrey.ac.uk
Stevens, Lisa M.
Crudgington, Bentley
Crooke, Helen R.
Werling, Dirk
Steinbach, Falkof.steinbach@surrey.ac.uk
Date : 15 August 2018
DOI : 10.3389/fimmu.2018.01800
Copyright Disclaimer : © 2018 Soldevila, Edwards, Graham, Stevens, Crudgington, Crooke, Werling and Steinbach. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Uncontrolled Keywords : Dendritic Cells; Macrophages, Myeloid; Porcine; Palatine Tonsil; Immunology; Pig
Depositing User : Clive Harris
Date Deposited : 25 Jul 2018 11:44
Last Modified : 17 Aug 2018 07:17
URI: http://epubs.surrey.ac.uk/id/eprint/848778

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