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In situ monitoring and analysis of enamel demineralisation using synchrotron X-ray scattering

Sui, Tan, Salvati, Enrico, Harper, Robert A, Zhang, Hongjia, Shelton, Richard M, Landini, Gabriel and Korsunsky, Alexander M (2018) In situ monitoring and analysis of enamel demineralisation using synchrotron X-ray scattering Acta Biomaterialia, 77. pp. 333-341.

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Abstract

Dental caries is one of the most common chronic diseases that affect human teeth. It often initiates in enamel, undermining its mechanical function and structural integrity. Little is known about the enamel demineralisation process caused by dental caries in terms of the microstructural changes and crystallography of the inorganic mineral phase. To improve the understanding of the carious lesion formation process and to help identify efficient treatments, the evolution of the microstructure at the nano-scale in an artificially induced enamel erosion region was probed using advanced synchrotron small-angle and wide-angle X-ray scattering (SAXS and WAXS). This is the first in vitro and time-resolved investigation of enamel demineralisation using synchrotron X-ray techniques which allows in situ quantification of the microstructure evolution over time in a simulated carious lesion. The analysis revealed that alongside the reduction of mineral volume, a heterogeneous evolution of hydroxyapatite (HAp) crystallites (in terms of size, preferred orientation and degree of alignment) could be observed. It was also found that the rate and direction of dissolution depends on the crystallographic orientation. Based on these findings, a novel conceptual view of the process is put forward that describes the key structural parameters in establishing high fidelity ultrastructure-based numerical models for the simulation of the enamel demineralisation process.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Mechanical Engineering Sciences
Authors :
NameEmailORCID
Sui, Tant.sui@surrey.ac.uk
Salvati, Enrico
Harper, Robert A
Zhang, Hongjia
Shelton, Richard M
Landini, Gabriel
Korsunsky, Alexander M
Date : 1 September 2018
Funders : EPSRC
DOI : 10.1016/j.actbio.2018.07.027
Copyright Disclaimer : © 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Uncontrolled Keywords : Dental caries Dental demineralisation Enamel SAXS WAXS in situ analysis
Depositing User : Melanie Hughes
Date Deposited : 19 Jul 2018 10:04
Last Modified : 18 Jul 2019 02:08
URI: http://epubs.surrey.ac.uk/id/eprint/848752

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