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The effect of some antioxidants in the process of ageing.

Rudra, Dwijendra Nath. (1976) The effect of some antioxidants in the process of ageing. Doctoral thesis, University of Surrey (United Kingdom)..

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Abstract

The effects of the natural antioxidant vitamin E and synthetic antioxidant ethoxyquin on the ageing process were studied in rats from 21 to 702 days after birth. Two dose levels of vitamin E, 0.05% and 0.5%, and ethoxyquin 0.5%, were used in the diet. In the long term study, the body weight gain of animals receiving diets containing both 0.05% and 0.5% vitamin E were greater than those of either pair-fed or ad-libitum fed controls and this suggests that the control diet was suboptimal with respect to its vitamin E content although the symptoms of vitamin E deficiency were not present. Early reduction in body weight gain in the 0.5% ethoxyquin-treated group was due to impaired palatability rather than impaired food utilisation because a similar growth rate was seen in the pair-fed controls. Later, however, the fall in the food intake and body weight correlated with the occurence of kidney damage. The liver and kidney weight of ethoxyquin-treated animals were significantly higher than in pair-fed controls. The kidney of the ethoxyquin-treated animals in the long term study showed chronic nephritis. In the short term study of two months, 0.5% ethoxyquin in the diet did not show nephrotoxicity. No abnormalities were seen in the kidneys of animals receiving vitamin E in the diet. The histology of bones of rats fed ethoxyquin for a long time suggested less deposition of calcium. In the short term study the humerus of ethoxyquin-fed rats contained a significantly lower level of calcium than in pair-fed controls and the bone weight was also significantly lower. In the long term study of antioxidants, both 0.5% vitamin E and ethoxyquin in the diet caused a significant reduction in PCV and a significant increase in the circulating reticulocytes. Ethoxyquin also significantly increased the clotting time. In the short term study, animals receiving the diets containing 0.05% vitamin E and 0.5% ethoxyquin contained significantly more reticulocytes than pair-fed controls. The clotting time was higher in antioxidant-treated animals, but the difference only reached statistical significance in the case of the 0.5% vitamin E treated animals. The accumulation of lipofuscin pigment in the brain of rats in the long term study shoved a significantly lover intensity of fluorescence in animals that received both 0.5% vitamin E and ethoxyquin in the diet, in spite of the fact that the entry of [14]C ethoxyquin into the brain seemed to be small. The possible relevance of this work to longevity and the ageing process is discussed.

Item Type: Thesis (Doctoral)
Divisions : Theses
Authors :
NameEmailORCID
Rudra, Dwijendra Nath.
Date : 1976
Contributors :
ContributionNameEmailORCID
http://www.loc.gov/loc.terms/relators/THS
Additional Information : Thesis (Ph.D.)--University of Surrey (United Kingdom), 1976.
Depositing User : EPrints Services
Date Deposited : 22 Jun 2018 14:25
Last Modified : 06 Nov 2018 16:53
URI: http://epubs.surrey.ac.uk/id/eprint/847969

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