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Production and study of human parathyroid hormone antibodies.

Mohamed, Ibrahim Ali. (1988) Production and study of human parathyroid hormone antibodies. Doctoral thesis, University of Surrey (United Kingdom)..

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Abstract

In this study, the immunogenicity of hPTH(1-34) was assessed in mice and rats, using different routes of immunization and two forms of hPTH(1-34), free and conjugated. The responder rats and mice were used for the production of monoclonal antibodies to hPTH(1-34). Several mouse-mouse and rat-mouse hybridoma cell lines were produced. The antibodies secreted were examined for various characteristics and used for the detection of hPTH(1-34) in biological specimens. Polyclonal antibody was also produced in goat against the free hPTH(1-34). Lymphocytes isolated from goat blood were thereafter used in the production of monoclonal antibodies. Fusion of goat B-lymphocytes with both mouse NS1 myeloma cell line and heteromyeloma (ovine-murine hybridoma) resulted in five cell lines secreting goat monoclonal antibodies to hPTH(1-34).

Item Type: Thesis (Doctoral)
Divisions : Theses
Authors :
NameEmailORCID
Mohamed, Ibrahim Ali.
Date : 1988
Contributors :
ContributionNameEmailORCID
http://www.loc.gov/loc.terms/relators/THS
Additional Information : Thesis (Ph.D.)--University of Surrey (United Kingdom), 1988.
Depositing User : EPrints Services
Date Deposited : 22 Jun 2018 14:24
Last Modified : 06 Nov 2018 16:53
URI: http://epubs.surrey.ac.uk/id/eprint/847814

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