University of Surrey

Test tubes in the lab Research in the ATI Dance Research

High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions

Phillips, Jack O, Butt, Louise E, Henderson, Charlotte A, Devonshire, Martin, Healy, Jess, Conway, Stuart J, Locker, Nicolas, Pickford, Andrew R, Vincent, Helen A and Callaghan, Anastasia J (2018) High-density functional-RNA arrays as a versatile platform for studying RNA-based interactions Nucleic Acids Research, 46 (14).

[img]
Preview
Text
High-density functional-RNA arrays.pdf - Version of Record
Available under License Creative Commons Attribution.

Download (3MB) | Preview

Abstract

We are just beginning to unravel the myriad of interactions in which non-coding RNAs participate. The intricate RNA interactome is the foundation of many biological processes, including bacterial virulence and human disease, and represents unexploited resources for the development of potential therapeutic interventions. However, identifying specific associations of a given RNA from the multitude of possible binding partners within the cell requires robust high-throughput systems for their rapid screening. Here, we present the first demonstration of functional-RNA arrays as a novel platform technology designed for the study of such interactions using immobilized, active RNAs. We have generated high-density RNA arrays by an innovative method involving surface-capture of in vitro transcribed RNAs. This approach has significant advantages over existing technologies, particularly in its versatility in regards to binding partner character. Indeed, proof-of-principle application of RNA arrays to both RNA–small molecule and RNA–RNA pairings is demonstrated, highlighting their potential as a platform technology for mapping RNA-based networks and for pharmaceutical screening. Furthermore, the simplicity of the method supports greater user-accessibility over currently available technologies. We anticipate that functional-RNA arrays will find broad utility in the expanding field of RNA characterization.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine
Authors :
NameEmailORCID
Phillips, Jack O
Butt, Louise E
Henderson, Charlotte A
Devonshire, Martin
Healy, Jess
Conway, Stuart J
Locker, NicolasN.Locker@surrey.ac.uk
Pickford, Andrew R
Vincent, Helen A
Callaghan, Anastasia J
Date : 28 May 2018
Funders : Biotechnology and Biological Sciences Research Council (BBSRC)
DOI : 10.1093/nar/gky410
Copyright Disclaimer : © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. Supplementary data
Uncontrolled Keywords : Protein-nucleic acid interaction; RNA characterisation and manipulation
Depositing User : Clive Harris
Date Deposited : 07 Jun 2018 12:10
Last Modified : 11 Dec 2018 11:24
URI: http://epubs.surrey.ac.uk/id/eprint/847011

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800