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Optical coherence tomography as a novel tool for non-destructive measurement of the hull thickness of lupin seeds

Clements, J.C., Zvyagin, A.V., Silva, K.K.M.B.D., Wanner, T., Sampson, David and Cowling, W.A. (2004) Optical coherence tomography as a novel tool for non-destructive measurement of the hull thickness of lupin seeds Plant Breeding, 123 (3). pp. 266-270.

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Abstract

Hull thickness is an important component of seed quality, which effects dehulling ability, feed or food nutritional aspects and cooking times. A breeding objective in Lupinus angustifolius crop improvement is to reduce hull thickness and a rapid screening method is needed to efficiently screen genotypes. Optical coherence tomography (OCT) imaging using infrared illumination at 980 nm was used to compare hull thickness of genotypes of four lupin species. OCT-derived hull layer thickness correlated highly with actual hull thickness determined by environmental scanning electron microscopy (r = 0.90) and allowed reliable distinction between mutant (thin-hulled) and parent genotypes of L. angustifolius. The imaging could clearly penetrate lupin seed to a depth of approximately 200 μm. The use of OCT to measure hull thickness has the advantage that it is rapid and non-destructive and should be very useful in selecting thin hull lines of lupins and other species on a single seed basis in germplasm or progeny from crosses.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Clements, J.C.
Zvyagin, A.V.
Silva, K.K.M.B.D.
Wanner, T.
Sampson, Davidd.sampson@surrey.ac.uk
Cowling, W.A.
Date : 2004
DOI : 10.1111/j.1439-0523.2004.00989.x
Uncontrolled Keywords : Hull, Lupinus, Optical coherence tomography, Scanning electron microscopy, Seed coat testa, Seed quality, Lupinus, Lupinus angustifolius
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 11 Jun 2018 09:12
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846926

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