Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents

Cicchi, R., Pavone, F.S., Massi, D. and Sampson, David (2005) Contrast and depth enhancement in two-photon microscopy of human skin ex vivo by use of optical clearing agents Optics Express, 13 (7). pp. 2337-2344.

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Abstract

We investigate the application of hyperosmotic optical clearing agents to improve the image contrast and penetration depth in two-photon microscopy of human dermis ex vivo. We show that the agents glycerol, propylene glycol, and glucose all convey significant improvements and we provide results on their dynamic behaviour and the reversibility of the effect. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging.

Item Type:	Article
Divisions :	Faculty of Engineering and Physical Sciences Faculty of Health and Medical Sciences
Authors :	Name Email ORCID Cicchi, R. Pavone, F.S. Massi, D. Sampson, David d.sampson@surrey.ac.uk
Date :	2005
DOI :	10.1364/OPEX.13.002337
Uncontrolled Keywords :	Acetic acid, Concentration (process), Fluorescence, Glycols, Light propagation, Medical imaging, Multiphoton processes, Photons, Propylene, Second harmonic generation, Tissue, Hyperosmotic agents, Light propagation in tissues, Optical clearing agents (OCA), Two-Photon microscopy, Skin
Depositing User :	Maria Rodriguez-Marquez
Date Deposited :	11 Jun 2018 09:02
Last Modified :	19 Sep 2018 11:32
URI:	http://epubs.surrey.ac.uk/id/eprint/846922

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