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Use of optical clearing agents in human dermis imaging by two photon microscopy

Cicchi, R., Sampson, David, Massi, D. and Pavone, F.S. (2005) Use of optical clearing agents in human dermis imaging by two photon microscopy

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Abstract

We investigate the effects arising from the topical application of optical clearing agents (OCA) in two photon microscopy imaging of human ex-vivo dermis tissue. We demonstrate that hyperosmotic agents such glycerol, propylene glycol and glucose in aqueous solution are effective in improving penetration depth and enhancing image contrast. We examine the results provided by the three agents, as well as the dynamical behaviour of the clarifying effect. Our results show that propylene glycol and anhydrous glycerol are more effective than glucose in enhancing contrast, also if glucose diffuses three and live times faster, respectively. All results are in agrement with a diffusive model of the agent into the tissue. At suitable concentrations, such agents have the potential to be compatible with living tissue and may possibly enhance in-vivo deep-tissue imaging.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Cicchi, R.
Sampson, Davidd.sampson@surrey.ac.uk
Massi, D.
Pavone, F.S.
Date : 2005
DOI : 586004
Uncontrolled Keywords : Contrast enhancement, Light propagation in tissue, Multiphoton microscopy, Optical clearing agent, Dynamic response, Glucose, Glycerol, Image quality, Microscopic examination, Multiphoton processes, Solutions, Contrast enhancement, Light propagation in tissue, Multiphoton microscopy, Optical clearing agents, Medical imaging
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 11 Jun 2018 09:04
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846916

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