Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy

Hillman, T.R., Alexandrov, S.A., Gutzler, T. and Sampson, David (2006) Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy Optics Express, 14 (23). pp. 11088-11102.

Full text not available from this repository.

Abstract

We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view.

Item Type:	Article
Divisions :	Faculty of Engineering and Physical Sciences Faculty of Health and Medical Sciences
Authors :	Name Email ORCID Hillman, T.R. Alexandrov, S.A. Gutzler, T. Sampson, David d.sampson@surrey.ac.uk
Date :	2006
DOI :	10.1364/OE.14.011088
Uncontrolled Keywords :	Fourier optics, Holography, Image analysis, Morphology, Spectroscopic analysis, Image captures, Image tiling, Mie theory predictions, Spherical scatterers, Light scattering
Depositing User :	Maria Rodriguez-Marquez
Date Deposited :	11 Jun 2018 08:54
Last Modified :	19 Sep 2018 11:32
URI:	http://epubs.surrey.ac.uk/id/eprint/846914

Actions (login required)

View Item

Downloads