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Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy

Hillman, T.R., Alexandrov, S.A., Gutzler, T. and Sampson, David (2006) Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy Optics Express, 14 (23). pp. 11088-11102.

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Abstract

We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Hillman, T.R.
Alexandrov, S.A.
Gutzler, T.
Sampson, Davidd.sampson@surrey.ac.uk
Date : 2006
DOI : 10.1364/OE.14.011088
Uncontrolled Keywords : Fourier optics, Holography, Image analysis, Morphology, Spectroscopic analysis, Image captures, Image tiling, Mie theory predictions, Spherical scatterers, Light scattering
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 11 Jun 2018 08:54
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846914

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