Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy
Hillman, T.R., Alexandrov, S.A., Gutzler, T. and Sampson, David (2006) Microscopic particle discrimination using spatially-resolved Fourier-holographic light scattering angular spectroscopy Optics Express, 14 (23). pp. 11088-11102.
Full text not available from this repository.Abstract
We utilize Fourier-holographic light scattering angular spectroscopy to record the spatially resolved complex angular scattering spectra of samples over wide fields of view in a single or few image captures. Without resolving individual scatterers, we are able to generate spatially-resolved particle size maps for samples composed of spherical scatterers, by comparing generated spectra with Mie-theory predictions. We present a theoretical discussion of the fundamental principles of our technique and, in addition to the sphere samples, apply it experimentally to a biological sample which comprises red blood cells. Our method could possibly represent an efficient alternative to the time-consuming and laborious conventional procedure in light microscopy of image tiling and inspection, for the characterization of microscopic morphology over wide fields of view.
Item Type: | Article | |||||||||||||||
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Divisions : |
Faculty of Engineering and Physical Sciences Faculty of Health and Medical Sciences |
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Authors : |
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Date : | 2006 | |||||||||||||||
DOI : | 10.1364/OE.14.011088 | |||||||||||||||
Uncontrolled Keywords : | Fourier optics, Holography, Image analysis, Morphology, Spectroscopic analysis, Image captures, Image tiling, Mie theory predictions, Spherical scatterers, Light scattering | |||||||||||||||
Depositing User : | Maria Rodriguez-Marquez | |||||||||||||||
Date Deposited : | 11 Jun 2018 08:54 | |||||||||||||||
Last Modified : | 19 Sep 2018 11:32 | |||||||||||||||
URI: | http://epubs.surrey.ac.uk/id/eprint/846914 |
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