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Deep-tissue access with confocal fluorescence microendoscopy through hypodermic needles

Pillai, R.S., Lorenser, D. and Sampson, D.D. (2011) Deep-tissue access with confocal fluorescence microendoscopy through hypodermic needles Optics Express, 19 (8). pp. 7213-7221.

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We report on the design and implementation of a gradient-index microendoscope suitable for accessing tissues deep within the body using confocal fluorescence imaging. The 350-μm diameter microendoscope has a length of 27 mm, which enables it to be inserted through a 22-gauge hypodermic needle. A prototype imaging system is demonstrated to obtain images of tissue samples at depths of ~15 mm with a lateral resolution of ~700 nm. To the best of our knowledge, this is the highest resolution and imaging depth reported for a confocal probe of these dimensions. We employ a scanning arrangement using a lensed fiber that can conveniently control the input beam parameters without causing off-axis aberrations typically present in the optical relay lenses used in galvanometer-mirror scanning systems.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
Pillai, R.S.
Lorenser, D.
Date : 2011
DOI : 10.1364/OE.19.007213
Uncontrolled Keywords : Fluorescence, Geometrical optics, Imaging systems, Lenses, Needles, Beam parameter, Confocal fluorescence, Confocal fluorescence imaging, Gradient indexes, Highest resolutions, Hypodermic needles, Imaging depth, Lateral resolution, Lensed fibers, Micro-endoscopy, Off-axis, Optical relay, Scanning systems, Tissue samples, Tissue, glass fiber, animal, article, cattle, confocal microscopy, endoscopy, equipment design, fluorescence microscopy, image processing, methodology, needle, normal distribution, optics, statistical model, Animals, Cattle, Endoscopy, Equipment Design, Image Processing, Computer-Assisted, Microscopy, Confocal, Microscopy, Fluorescence, Models, Statistical, Needles, Normal Distribution, Optical Fibers, Optics and Photonics
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 06 Jun 2018 10:54
Last Modified : 19 Sep 2018 11:32

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