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Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe

Pillai, R.S., Lorenser, D. and Sampson, D.D. (2011) Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe

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Abstract

We report on the design and implementation of a 350 μm-diameter confocal imaging probe based on gradient-index (GRIN) optics and a fiber-based scanning arrangement. The form factor of the probe is such that it can potentially be inserted into a 22-gauge hypodermic needle to perform high-resolution confocal fluorescence imaging in solid tissues. We introduce a simple scanning arrangement based on lensed fiber, which eliminates off-axis aberrations induced by conventional scanning optics and is suitable for integration into a compact hand-held unit. We present the details of the optical design and experimental verification of the performance of the optical system. The measured lateral resolution of ~700 nm is in agreement with the optical design and is the highest resolution reported for a confocal fluorescence imaging probe of this size. Further, we demonstrate the imaging capability of the probe by obtaining high-resolution images of fluorescently labeled muscle fibers.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Pillai, R.S.
Lorenser, D.
Sampson, D.D.d.sampson@surrey.ac.uk
Date : 2011
DOI : 10.1117/12.887373
Uncontrolled Keywords : Confocal microscopy, fluorescence, gradient-index fiber, in vivo imaging, needle probe, optical biopsy, Confocal fluorescence imaging, Confocal imaging, Experimental verification, Fiber-optic confocal microscopy, Form factors, Gradient indexes, Gradient-index, High resolution, High resolution image, Highest resolutions, Hypodermic needles, Imaging capabilities, Imaging probe, In-Vivo imaging, Lateral resolution, Lensed fibers, Muscle fiber, needle probe, Off-axis, Optical biopsies, Solid tissues, Approximation theory, Confocal microscopy, Fiber optic sensors, Fibers, Fluorescence, Needles, Optical design, Optical fibers, Optical systems, Probes, Scanning, Sensors, Tissue, Aberrations
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 06 Jun 2018 10:56
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846850

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