Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe
Pillai, R.S., Lorenser, D. and Sampson, D.D. (2011) Fiber-optic confocal microscopy using a miniaturized needle-compatible imaging probe
Full text not available from this repository.Abstract
We report on the design and implementation of a 350 μm-diameter confocal imaging probe based on gradient-index (GRIN) optics and a fiber-based scanning arrangement. The form factor of the probe is such that it can potentially be inserted into a 22-gauge hypodermic needle to perform high-resolution confocal fluorescence imaging in solid tissues. We introduce a simple scanning arrangement based on lensed fiber, which eliminates off-axis aberrations induced by conventional scanning optics and is suitable for integration into a compact hand-held unit. We present the details of the optical design and experimental verification of the performance of the optical system. The measured lateral resolution of ~700 nm is in agreement with the optical design and is the highest resolution reported for a confocal fluorescence imaging probe of this size. Further, we demonstrate the imaging capability of the probe by obtaining high-resolution images of fluorescently labeled muscle fibers.
Item Type: | Conference or Workshop Item (UNSPECIFIED) | ||||||||||||
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Divisions : |
Faculty of Engineering and Physical Sciences Faculty of Health and Medical Sciences |
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Authors : |
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Date : | 2011 | ||||||||||||
DOI : | 10.1117/12.887373 | ||||||||||||
Uncontrolled Keywords : | Confocal microscopy, fluorescence, gradient-index fiber, in vivo imaging, needle probe, optical biopsy, Confocal fluorescence imaging, Confocal imaging, Experimental verification, Fiber-optic confocal microscopy, Form factors, Gradient indexes, Gradient-index, High resolution, High resolution image, Highest resolutions, Hypodermic needles, Imaging capabilities, Imaging probe, In-Vivo imaging, Lateral resolution, Lensed fibers, Muscle fiber, needle probe, Off-axis, Optical biopsies, Solid tissues, Approximation theory, Confocal microscopy, Fiber optic sensors, Fibers, Fluorescence, Needles, Optical design, Optical fibers, Optical systems, Probes, Scanning, Sensors, Tissue, Aberrations | ||||||||||||
Depositing User : | Maria Rodriguez-Marquez | ||||||||||||
Date Deposited : | 06 Jun 2018 10:56 | ||||||||||||
Last Modified : | 19 Sep 2018 11:32 | ||||||||||||
URI: | http://epubs.surrey.ac.uk/id/eprint/846850 |
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