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Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography

Klyen, B.R., Shavlakadze, T., Radley-Crabb, H.G., Grounds, M.D. and Sampson, D.D. (2011) Identification of muscle necrosis in the mdx mouse model of Duchenne muscular dystrophy using three-dimensional optical coherence tomography Journal of Biomedical Optics, 16 (7).

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Abstract

Three-dimensional optical coherence tomography (3D-OCT) was used to image the structure and pathology of skeletal muscle tissue from the treadmill-exercised mdx mouse model of human Duchenne muscular dystrophy. Optical coherence tomography (OCT) images of excised muscle samples were compared with coregistered hematoxylin and eosin-stained and Evans blue dye fluorescence histology. We show, for the first time, structural 3D-OCT images of skeletal muscle dystropathology well correlated with co-located histology. OCT could identify morphological features of interest and necrotic lesions within the muscle tissue samples based on intrinsic optical contrast. These findings demonstrate the utility of 3D-OCT for the evaluation of small-animal skeletal muscle morphology and pathology, particularly for studies of mouse models of muscular dystrophy.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Klyen, B.R.
Shavlakadze, T.
Radley-Crabb, H.G.
Grounds, M.D.
Sampson, D.D.d.sampson@surrey.ac.uk
Date : 2011
DOI : 10.1117/1.3598842
Uncontrolled Keywords : Duchenne muscular dystrophy, Evans blue dye, Mdx mouse, Necrotic lesion, Optical coherence tomography, Skeletal muscle, Small-animal imaging, Three-dimensional imaging, Evans blue dyes, Mdx mouse, Muscular dystrophy, Necrotic lesion, Skeletal muscle, Small animal imaging, Three dimensional imaging, Diseases, Histology, Mammals, Muscle, Neuromuscular rehabilitation, Pathology, Tomography, Three dimensional, Animalia, coloring agent, diagnostic agent, Evans blue, animal, article, disease model, Duchenne muscular dystrophy, evaluation, human, instrumentation, male, methodology, mouse, necrosis, optical coherence tomography, pathology, skeletal muscle, three dimensional imaging, X chromosome linked muscular dystrophic mouse, Animals, Coloring Agents, Disease Models, Animal, Evans Blue, Humans, Imaging, Three-Dimensional, Male, Mice, Mice, Inbred mdx, Muscle, Skeletal, Muscular Dystrophy, Duchenne, Necrosis, Tomography, Optical Coherence
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 05 Jun 2018 15:56
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846849

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