Molecular imaging needles: Dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue

Scolaro, L., Lorenser, D., Madore, W.-J., Kirk, R.W., Kramer, A.S., Yeoh, G.C., Godbout, N., Sampson, David, Boudoux, C. and McLaughlin, R.A. (2015) Molecular imaging needles: Dual-modality optical coherence tomography and fluorescence imaging of labeled antibodies deep in tissue Biomedical Optics Express, 6 (5). pp. 1767-1781.

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Abstract

Molecular imaging using optical techniques provides insight into disease at the cellular level. In this paper, we report on a novel dualmodality probe capable of performing molecular imaging by combining simultaneous three-dimensional optical coherence tomography (OCT) and two-dimensional fluorescence imaging in a hypodermic needle. The probe, referred to as a molecular imaging (MI) needle, may be inserted tens of millimeters into tissue. The MI needle utilizes double-clad fiber to carry both imaging modalities, and is interfaced to a 1310-nm OCT system and a fluorescence imaging subsystem using an asymmetrical double-clad fiber coupler customized to achieve high fluorescence collection efficiency. We present, to the best of our knowledge, the first dual-modality OCT and fluorescence needle probe with sufficient sensitivity to image fluorescently labeled antibodies. Such probes enable high-resolution molecular imaging deep within tissue.

Item Type:	Article
Divisions :	Faculty of Engineering and Physical Sciences Faculty of Health and Medical Sciences
Authors :	Name Email ORCID Scolaro, L. Lorenser, D. Madore, W.-J. Kirk, R.W. Kramer, A.S. Yeoh, G.C. Godbout, N. Sampson, David d.sampson@surrey.ac.uk Boudoux, C. McLaughlin, R.A.
Date :	2015
DOI :	10.1364/BOE.6.001767
Uncontrolled Keywords :	Antibodies, Fluorescence, Needles, Optical tomography, Probes, Tissue, Tomography, Cellular levels, Collection efficiency, Double clad fibers, Dual-modality probes, Fluorescence imaging, Hypodermic needles, Imaging modality, Optical technique, Molecular imaging, adult, animal cell, animal tissue, antibody labeling, Article, controlled study, fluorescence imaging, human, human cell, human tissue, imaging system, immunofluorescence, molecular imaging, mouse, nonhuman, optical coherence tomography, sensitivity analysis, signal detection, signal noise ratio, three dimensional imaging, tissues
Depositing User :	Maria Rodriguez-Marquez
Date Deposited :	05 Jun 2018 08:40
Last Modified :	19 Sep 2018 11:32
URI:	http://epubs.surrey.ac.uk/id/eprint/846777

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