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Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

Curatolo, A., Villiger, M., Lorenser, D., Wijesinghe, P., Fritz, A., Kennedy, B.F. and Sampson, David (2016) Ultrahigh resolution optical coherence elastography using a Bessel beam for extended depth of field

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Abstract

Visualizing stiffness within the local tissue environment at the cellular and sub-cellular level promises to provide insight into the genesis and progression of disease. In this paper, we propose ultrahigh-resolution optical coherence elastography, and demonstrate three-dimensional imaging of local axial strain of tissues undergoing compressive loading. The technique employs a dual-arm extended focus optical coherence microscope to measure tissue displacement under compression. The system uses a broad bandwidth supercontinuum source for ultrahigh axial resolution, Bessel beam illumination and Gaussian beam detection, maintaining sub-2 μm transverse resolution over nearly 100 μm depth of field, and spectral-domain detection allowing high displacement sensitivity. The system produces strain elastograms with a record resolution (x,y,z) of 2×2×15 μm. We benchmark the advances in terms of resolution and strain sensitivity by imaging a suitable inclusion phantom. We also demonstrate this performance on freshly excised mouse aorta and reveal the mechanical heterogeneity of vascular smooth muscle cells and elastin sheets, otherwise unresolved in a typical, lower resolution optical coherence elastography system.

Item Type: Conference or Workshop Item (UNSPECIFIED)
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Curatolo, A.
Villiger, M.
Lorenser, D.
Wijesinghe, P.
Fritz, A.
Kennedy, B.F.
Sampson, Davidd.sampson@surrey.ac.uk
Date : 2016
DOI : 10.1117/12.2214684
Uncontrolled Keywords : Image enhancement, Laser beam shaping, Optical coherence tomography, Tissue characterization, Bessel functions, Gaussian beams, Image enhancement, Laser beams, Medical imaging, Optical tomography, Tomography, Extended depth of field, Laser beam shaping, Mechanical heterogeneity, Optical coherence elastography, Supercontinuum sources, Three dimensional imaging, Tissue characterization, Vascular Smooth Muscle Cells, Tissue
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 04 Jun 2018 09:11
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846769

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