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Deep tissue volume imaging of birefringence through fibre-optic needle probes for the delineation of breast tumour

Villiger, M., Lorenser, D., McLaughlin, R.A., Quirk, B.C., Kirk, R.W., Bouma, B.E. and Sampson, David (2016) Deep tissue volume imaging of birefringence through fibre-optic needle probes for the delineation of breast tumour Scientific Reports, 6.

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Abstract

Identifying tumour margins during breast-conserving surgeries is a persistent challenge. We have previously developed miniature needle probes that could enable intraoperative volume imaging with optical coherence tomography. In many situations, however, scattering contrast alone is insufficient to clearly identify and delineate malignant regions. Additional polarization-sensitive measurements provide the means to assess birefringence, which is elevated in oriented collagen fibres and may offer an intrinsic biomarker to differentiate tumour from benign tissue. Here, we performed polarization-sensitive optical coherence tomography through miniature imaging needles and developed an algorithm to efficiently reconstruct images of the depth-resolved tissue birefringence free of artefacts. First ex vivo imaging of breast tumour samples revealed excellent contrast between lowly birefringent malignant regions, and stromal tissue, which is rich in oriented collagen and exhibits higher birefringence, as confirmed with co-located histology. The ability to clearly differentiate between tumour and uninvolved stroma based on intrinsic contrast could prove decisive for the intraoperative assessment of tumour margins.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Villiger, M.
Lorenser, D.
McLaughlin, R.A.
Quirk, B.C.
Kirk, R.W.
Bouma, B.E.
Sampson, Davidd.sampson@surrey.ac.uk
Date : 2016
DOI : 10.1038/srep28771
Uncontrolled Keywords : algorithm, birefringence, breast, breast tumor, devices, diagnostic imaging, female, fiber optics, human, intraoperative period, needle, optical coherence tomography, pathology, polarization microscopy, procedures, reproducibility, Algorithms, Birefringence, Breast, Breast Neoplasms, Female, Fiber Optic Technology, Humans, Intraoperative Period, Microscopy, Polarization, Needles, Reproducibility of Results, Tomography, Optical Coherence
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 04 Jun 2018 09:55
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846761

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