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Ultrahigh-resolution optical coherence elastography through a micro-endoscope: Towards in vivo imaging of cellular-scale mechanics

Fang, Q., Curatolo, A., Wijesinghe, P., Yeow, Y.L., Hamzah, J., Noble, P.B., Karnowski, K., Sampson, David, Ganss, R., Kim, J.K. , Lee, W.M. and Kennedy, B.F. (2017) Ultrahigh-resolution optical coherence elastography through a micro-endoscope: Towards in vivo imaging of cellular-scale mechanics Biomedical Optics Express, 8 (11). pp. 5127-5138.

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Abstract

In this paper, we describe a technique capable of visualizing mechanical properties at the cellular scale deep in living tissue, by incorporating a gradient-index (GRIN)-lens micro-endoscope into an ultrahigh-resolution optical coherence elastography system. The optical system, after the endoscope, has a lateral resolution of 1.6 µm and an axial resolution of 2.2 µm. Bessel beam illumination and Gaussian mode detection are used to provide an extended depth-of-field of 80 µm, which is a 4-fold improvement over a fully Gaussian beam case with the same lateral resolution. Using this system, we demonstrate quantitative elasticity imaging of a soft silicone phantom containing a stiff inclusion and a freshly excised malignant murine pancreatic tumor. We also demonstrate qualitative strain imaging below the tissue surface on in situ murine muscle. The approach we introduce here can provide high-quality extended-focus images through a micro-endoscope with potential to measure cellular-scale mechanics deep in tissue. We believe this tool is promising for studying biological processes and disease progression in vivo.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences
Faculty of Health and Medical Sciences
Authors :
NameEmailORCID
Fang, Q.
Curatolo, A.
Wijesinghe, P.
Yeow, Y.L.
Hamzah, J.
Noble, P.B.
Karnowski, K.
Sampson, Davidd.sampson@surrey.ac.uk
Ganss, R.
Kim, J.K.
Lee, W.M.
Kennedy, B.F.
Date : 2017
DOI : 10.1364/BOE.8.005127
Uncontrolled Keywords : Biomechanics, Endoscopy, Lenses, Medical imaging, Optical systems, Silicones, Tissue, Biological process, Disease progression, Elasticity imaging, Extended depth of field, Gradient index lens, Lateral resolution, Optical coherence elastography, Ultrahigh resolution, Gaussian beams, iron oxide, silicone, titanium dioxide, Article, breast tissue, disease course, elastography, endoscopy, image analysis, in vivo study, light intensity, multiphoton microscopy, nonhuman, optical coherence elastography, pancreas tumor, piezoelectricity, signal noise ratio, Young modulus
Depositing User : Maria Rodriguez-Marquez
Date Deposited : 04 Jun 2018 08:06
Last Modified : 19 Sep 2018 11:32
URI: http://epubs.surrey.ac.uk/id/eprint/846731

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