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Immunohistochemical characterization of tuberculous lesions in sheep naturally infected with Mycobacterium bovis

Vallejo, Raquel, García Marín, Juan F., Juste, Ramón A., Muñoz-Mendoza, Marta, Salguero, Francisco J. and Balseiro, Ana (2018) Immunohistochemical characterization of tuberculous lesions in sheep naturally infected with Mycobacterium bovis BMC Veterinary Research, 14 (154). pp. 1-7.

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Abstract

Background: Sheep have been traditionally considered as less susceptible to Mycobacterium bovis (Mbovis) infection than other domestic ruminants such as cattle and goats. However, there is increasing evidence for the role of this species as a domestic Mbovis reservoir, mostly when sheep share grazing fields with infected cattle and goats. Nevertheless, there is a lack of information about the pathogenesis and the immune response of Mbovis infection in sheep. The goals of this study were to characterize the granuloma stages produced by the natural infection of Mbovis in sheep, to compare them with other species and to identify possible differences in the sheep immune response. Samples from bronchial lymph nodes from twelve Mbovis-naturally infected sheep were used. Four immunohistochemical protocols for the specific detection of T-lymphocytes, B-lymphocytes, plasma cells and macrophages were performed to study the local immune reaction within the granulomas.

Results: Differences were observed in the predominant cell type present in each type of granuloma, as well as differences and similarities with the development of tuberculous granulomas in other species. Very low numbers of T-lymphocytes were observed in all granuloma types indicating that specific cellular immune response mediated by T-cells might not be of much importance in sheep in the early stages of infection, when macrophages are the predominant cell type within lesions. Plasma cells and mainly B lymphocytes increased considerably as the granuloma developed being attracted to the lesions in a shift towards a Th2 response against the increasing amounts of mycobacteria. Therefore, we have proposed that the granulomas could be defined as initial, developed and terminal.

Conclusions: Results showed that the study of the lymphoid tissue granulomata reinforces the view that the three different types of granuloma represent stages of lesion progression and suggest an explanation to the higher resistance of sheep based on a higher effective innate immune response to control tuberculosis infection.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Vallejo, Raquel
García Marín, Juan F.
Juste, Ramón A.
Muñoz-Mendoza, Marta
Salguero, Francisco J.f.salguerobodes@surrey.ac.uk
Balseiro, Ana
Date : 4 May 2018
DOI : 10.1186/s12917-018-1476-2
Copyright Disclaimer : © The Author(s). 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Uncontrolled Keywords : Tuberculosis; Sheep; Immunohistochemistry; Mycobacterium bovis; Granuloma
Related URLs :
Depositing User : Clive Harris
Date Deposited : 26 Apr 2018 10:51
Last Modified : 16 Jan 2019 19:09
URI: http://epubs.surrey.ac.uk/id/eprint/846309

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