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Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings

Ashpitel, H.F., Dabbs, E.B., Nemchand, J.L., La Ragione, R.M., Salguero Bodes, F.J. and Whiteley, M.S. (2018) Histological and Immunofluorescent Analysis of a Large Tributary of the Great Saphenous Vein Treated with a 1920 nm Endovenous Laser: Preliminary Findings EJVES Short Reports, 39. pp. 7-11.

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Abstract

Objectives: To analyse the biological effects of a 1920 nm endovenous laser (EVL) on extra-fascial great saphenous vein (GSV) in vitro.

Methods: A 10 cm length of a large tributary bypassing a hypoplastic segment of the GSV (sometimes called an “extra-fascial GSV”) was obtained during routine varicose vein surgery. The length was treated in five sections with different LEEDs (0 (control), 20, 40, 60, and 80 J/cm) with a 1920 nm EVL at 4W power, in a novel in vitro treatment model. The biological effects were assessed by histological staining of the samples for haematoxylin and eosin (HE) and Martius Scarlet Blue (MSB), and by immunofluorescent detection of p-p53 and VCAM-1.

Results: Histological analysis showed significant structural damage at LEEDs above 60 J/cm, especially in the intima and media, with the treatment at 80 J/cm causing perforation of the vein wall. In addition, there was a significant increase in p-p53 expression in treated tissue at 60 and 80 J/cm.

Conclusions: Using this ex vivo model, the results indicate that in vitro treatment with a 1920 nm EVL, at or above an LEED of 60 J/cm and 4 W power, causes significant vein wall cell death reaching deep into the media by a combination of direct thermal damage and apoptosis. A wavelength of 1920 nm appears to be effective for the endovenous ablation of truncal veins.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Ashpitel, H.F.h.ashpitel@surrey.ac.uk
Dabbs, E.B.
Nemchand, J.L.j.nemchand@surrey.ac.uk
La Ragione, R.M.R.Laragione@surrey.ac.uk
Salguero Bodes, F.J.f.salguerobodes@surrey.ac.uk
Whiteley, M.S.
Date : 18 April 2018
DOI : 10.1016/j.ejvssr.2018.03.003
Copyright Disclaimer : © 2018 The Author(s). Published by Elsevier Ltd on behalf of European Society for Vascular Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Uncontrolled Keywords : Endovenous thermal ablation; Endovenous laser treatment; Great saphenous vein; Histology; Immunofluorescence
Depositing User : Clive Harris
Date Deposited : 26 Apr 2018 09:01
Last Modified : 16 Jan 2019 19:09
URI: http://epubs.surrey.ac.uk/id/eprint/846304

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