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Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10

Singleton, Helen, Graham, Simon P., Frossard, Jean-Pierre, Bodman-Smith, Katherine B. and Steinbach, Falko (2018) Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10 Virology, 517. pp. 199-207.

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Abstract

Monocytes are considered refractory to porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) infection. However, monocytes are only short-lived in blood, being able to differentiate into macrophages and dendritic cells (DC). It was therefore merited to revisit PRRSV-1 interaction with monocytes, particularly those treated with cytokines influencing monocyte biology. Thus, several factors were screened, particularly those modulating monocyte differentiation and expression of putative PRRSV-1 receptors (CD169 and CD163). M-CSF, known to stimulate macrophage differentiation, did not increase their susceptibility to PRRSV-1. Nor did GM-CSF or IL-4, known drivers for monocyte-derived DC (MoDC) differentiation. In contrast, monocyte treatment with IL-10 or the corticosteroid, dexamethasone, known to be potent suppressors of monocyte differentiation, was correlated with increased susceptibility to PRRSV-1 infection. While this effect was strongly correlated to CD163 and CD169 expression, our data suggest that receptor expression is not the only factor driving successful infection of PPRSV-1 in monocytes.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
NameEmailORCID
Singleton, Helen
Graham, Simon P.s.graham@surrey.ac.uk
Frossard, Jean-Pierre
Bodman-Smith, Katherine B.K.Bodman-Smith@surrey.ac.uk
Steinbach, Falkof.steinbach@surrey.ac.uk
Date : 2 March 2018
Identification Number : 10.1016/j.virol.2018.02.017
Copyright Disclaimer : Crown Copyright © 2018 Published by Elsevier Inc. All rights reserved.
Uncontrolled Keywords : PRRSV-1; Monocytes; Dexamethasone; IL-10; Macrophages; Infection
Depositing User : Clive Harris
Date Deposited : 12 Apr 2018 15:01
Last Modified : 12 Apr 2018 15:22
URI: http://epubs.surrey.ac.uk/id/eprint/846209

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